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Quantification of DNA Damage Using Mass Spectrometry Techniques

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Nucleic Acids in the Gas Phase

Part of the book series: Physical Chemistry in Action ((PCIA))

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Abstract

The role of DNA in cells relies on its chemical structure. Unfortunately, a number of physical and chemical agents may damage DNA, leading to modification of genetic information or to cell death. Detecting DNA damage is thus a major issue in numerous studies. Analytical methods have therefore been developed in order to quantify modified DNA bases. In particular, mass spectrometry is used as a very specific and sensitive detector when combined to gas or liquid chromatography. This review will first briefly present the major classes of DNA damage and then focus on two mass spectrometry-based approaches for the quantification of modified bases following DNA hydrolysis into monomers.

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Abbreviations

64PPS:

6-4 Pyrimidine dimer photoproducts (35)

B(a)P:

Benzo[a]pyrene

BPDE:

Benzo(a)pyrene diol epoxide

CPDs:

Cyclobutane pyrimidine dimers (34)

DEW:

Pyrimidine dimer Dewar valence isomer (36)

EIMS:

Electron impact ionization mass spectrometry

GC:

Gas chromatography

HPLC:

High-performance liquid chromatography

LMDS:

Locally multiply damaged sites

MMS:

Methyl methanesulfonate

MS/MS:

Tandem mass spectrometry

MS:

Mass spectrometry

NCIMS:

Negative chemical ionization mass spectrometry

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  1. Laboratoire “Lésions des Acides Nucléiques”, Université Joseph Fourier – Grenoble 1/CEA/ Institut Nanoscience et Cryogénie/SCIB, UMR-E3, Grenoble, France

    Thierry Douki & Jean-Luc Ravanat

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  1. Thierry Douki
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  2. Jean-Luc Ravanat
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Correspondence to Thierry Douki .

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  1. Université de Bordeaux, Institut Européen de Chimie et Biologie, Pessac, France

    Valérie Gabelica

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Douki, T., Ravanat, JL. (2014). Quantification of DNA Damage Using Mass Spectrometry Techniques. In: Gabelica, V. (eds) Nucleic Acids in the Gas Phase. Physical Chemistry in Action. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54842-0_8

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