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Transferring Nucleic Acids to the Gas Phase

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Nucleic Acids in the Gas Phase

Part of the book series: Physical Chemistry in Action ((PCIA))

Abstract

During the past 30 years, tremendous efforts have been made to implement mass spectrometry and spectroscopy technologies for the characterization of biomolecules in the gas phase. Progresses in the study of gas phase oligonucleotides and DNA have come with the advent of different sources capable to transfer these fragile biomolecules from the condensed phase into the gas phase. These techniques have been largely employed in the spectroscopy and mass spectrometry communities and have stimulated much research with applications of mass spectrometry to structural biology and applications of spectroscopy to detailed understanding of the intra- and intermolecular interactions. The key point in all the experimental techniques is to counterpoise the extremely low volatility of nucleobases, oligonucleotides, and higher-order DNA structures while keeping these thermally fragile molecules intact. The aim of this chapter is to describe the transfer of nucleic acids to the gas phase, including the technical and experimental issues that have been successfully overcome over the last decades.

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Abbreviations

6-ATT:

6-Aza-2-thiothymine

CRM:

Charge residue model

DAHC:

Diammonium hydrogen citrate

DNA:

Deoxyribonucleic acid

ESI:

Electrospray ionization

FTICR:

Fourier transform ion cyclotron resonance analyzer

FWHM:

Full width at half maximum

HPA:

3-Hydroxypicolinic acid

HPLC:

High performance liquid chromatography

IEM:

Ion evaporation model

LILBID:

Laser-induced liquid beam(bead) ionization/desorption

MALD(I):

Matrix-assisted laser desorption (ionization)

MS:

Mass spectrometry

NB:

Nucleobases

NH4OAc:

Ammonium acetate

PA:

Picolinic acid

PCA:

Pyrazine carboxylic acid

reTOF:

Reflectron time-of-flight

RNA:

Ribonucleic acid

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  1. Laboratoire de Physique des Lasers, Université Paris 13, Sorbonne Paris Cité, 93430, Villetaneuse, France

    Gilles Grégoire

  2. CNRS, UMR 7538, LPL, 93430, Villetaneuse, France

    Gilles Grégoire

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  1. Gilles Grégoire
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  1. Université de Bordeaux, Institut Européen de Chimie et Biologie, Pessac, France

    Valérie Gabelica

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Grégoire, G. (2014). Transferring Nucleic Acids to the Gas Phase. In: Gabelica, V. (eds) Nucleic Acids in the Gas Phase. Physical Chemistry in Action. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-54842-0_2

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