Abstract
Ochratoxin A is a thermoresistant mycotoxin produced by ubiquitous molds of Aspergillus and Penicillium genera. It contaminates foodstuffs and feedstuffs worldwide and therefore is of human and animal concern.
Ochratoxin A induces oxidative stress, inflammation, and fibrosis, and is nephrotoxic, hepatotoxic, and neurotoxicin particularly in male subjects. Toxicity is mainly exerted through epigenetic mechanisms.
Nephrotoxicity is probably due to ochratoxin A-induced suppression of the collagen regulator mir-29b that results in an increase of translated collagen, fibrotic alteration, and nephropathy. Alternatively, ochratoxin A induces mir-132 upregulation that occurs in neurologic and psychiatric conditions as well as in oxidative stress. Undeniably, mir-132 acts in the reciprocal regulation of autism-related genes MeCP2 and PTEN decreasing the antioxidant Nrf2 that leads to the formation of high levels of reactive oxygen species. Reactive oxygen species, in turn, enhance the expression of mir-200c that impairs antioxidative mechanisms and synaptic plasticity through the reduction of HO-1 and NLGN4X. As for apoptosis, OTA exposure increases mir-122 that suppresses the anti-apoptotic genes Bcl-w and caspase-3 leading to cell death and hepatic damage.
Interestingly, both MECP2 and NLGN4X are involved in neurodevelopmental disorders, including autism, and are mapped on the X chromosome. As autism is a male predominant disorder, a possible contribution of ochratoxin A in its pathogenesis and in its strong male bias can be suggested.
Very few papers report about ochratoxin A-induced deacetylation:cells exposed to OTA underwent to a dramatic block of histone acetyltransferases leading to mitotic arrest and Nrf2 inhibition that, again, lead to reactive oxygen species formation.
Further studies are needed to obtain a complete picture of ochratoxin A-dependent epigenetic effects and to prevent or to counteract them.
Abbreviations
- AREs:
-
Antioxidant responsive elements
- ASD:
-
Autism spectrum disorder (ASD)
- BACE1:
-
β-Secretase-1 enzyme
- BBB:
-
Blood brain barrier
- BDNF :
-
Brain-derived neurotropic factor
- BEN:
-
Balkan endemic nephropathy
- CASP3 :
-
Caspase3
- CBP:
-
CREB-binding protein
- CNS:
-
Central nervous system
- DGCR8 :
-
DiGeorge syndrome critical region gene 8
- FMRP:
-
Fragile X mental retardation protein
- HAT:
-
Histone acetyltransferase
- HDAC:
-
Histone deacetylase
- HO-1 :
-
Heme oxygenase-1
- MeCP2 :
-
Methyl-CpG-binding protein 2
- NLGN4X :
-
Neuroligin4x
- OTA:
-
Ochratoxin A
- p300:
-
Adenoviral E1A-associated protein
- PAH :
-
Phenylalanine hydroxylase
- phe:
-
Phenylalanine
- PKU:
-
Phenylketonuria
- PTEN :
-
Phosphatase and tensin homolog
- TGFβ:
-
Transforming-growth factor-beta
- Nrf2 :
-
Nuclear factor erythroid 2-like 2
- ROS:
-
Reactive oxygen species
- tyr:
-
tyrosine
- ZEB1 :
-
Zinc finger E-box binding homeobox 1
References
Baieli S, Pavone L, Meli C et al (2003) Autism and phenylketonuria. J Autism Dev Disord 33(2):201–204
Baskerville TA, Douglas AJ (2010) Dopamine and oxytocin interactions underlying behaviors: potential contributions to behavioral disorders. CNS Neurosci Ther 16(3):e92–123. doi:10.1111/j.1755-5949.2010.00154.x. Review. PubMed PMID: 20557568
Baudrimont I, Sostaric B, Yenot C, Betbeder AM, Dano-Djedje S, Sanni A, Steyn PS, Creppy EE (2001) Aspartame prevents the karyomegaly induced by ochratoxin A in rat kidney. Arch Toxicol 75(3):176–83
Bemben MA, Nguyen QA, Wang T et al (2015) Autism-associated mutation inhibits protein kinase C-mediated neuroligin-4X enhancement of excitatory synapses. Proc Natl Acad Sci U S A 112(8):2551–2556. doi:10.1073/pnas.1500501112
Beveridge NJ, Gardiner E, Carroll AP et al (2010) Schizophrenia is associated with an increase in cortical microRNA biogenesis. Mol Psychiatry 15(12):1176–1189. doi:10.1038/mp.2009.84
Bhat PV, Md P, Khanum F et al (2016) Cytotoxic effects of ochratoxin A in neuro-2a cells: role of oxidative stress evidenced by N-acetylcysteine. Front Microbiol 7:1142. doi:10.3389/fmicb.2016.01142
Boudra H, Le Bars P, Le Bars J (1995) Thermostability of ochratoxin A in wheat under two moisture conditions. Appl Environ Microbiol 61:1156–1158
Caccamo A, Maldonado MA, Bokov AF et al (2010) CBP gene transfer increases BDNF levels and ameliorates learning and memory deficits in a mouse model of Alzheimer’s disease. Proc Natl Acad Sci U S A 107:22687–22692. doi:10.1073/pnas.1012851108
Cai G, Edelmann L, Goldsmith JE et al (2008) Multiplex ligation-dependent probe amplification for genetic screening in autism spectrum disorders: efficient identification of known microduplications and identification of a novel microduplication in ASMT. BMC Med Genet 1:50. doi:10.1186/1755-8794-1-50
Castegnaro M, Canadas D, Vrabcheva T et al (2006) Balkan endemic nephropathy: role of ochratoxins A through biomarkers. Mol Nutr Food Res 50(6):519–529
Cheng TL, Wang Z, Liao Q et al (2014) MeCP2 suppresses nuclear microRNA processing and dendritic growth by regulating the DGCR8/Drosha complex. Dev Cell 28(5):547–560. doi:10.1016/j.devcel.2014.01.032
Chou C, Chang N, Shrestha S, Hsu S, Lin Y, Lee W et al (2015) miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database. Nucleic Acids Res 44(D1):D239–D247
Choudhary C, Kumar C, Gnad F et al (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science 325(5942):834–840. doi:10.1126/science.1175371
Clevers H (2006) Wnt/beta-catenin signaling in development and disease. Cell 127:469–480
Creppy EE, Chakor K, Fisher MJ et al (1990) The myocotoxin ochratoxin A is a substrate for phenylalanine hydroxylase in isolated rat hepatocytes and in vivo. Arch Toxicol 64(4):279–284
Cuadrado-Tejedor M, Vilariño M, Cabodevilla F et al (2011) Enhanced expression of the voltage-dependent anion channel 1 (VDAC1) in Alzheimer’s disease transgenic mice: an insight into the pathogenic effects of amyloid-β. J Alzheimers Dis 23(2):195–206. doi:10.3233/JAD-2010-100966
Czakai K, Müller K, Mosesso P et al (2011) Perturbation of mitosis through inhibition of histone acetyltransferases: the key to ochratoxin a toxicity and carcinogenicity? Toxicol Sci 122(2):317–329. doi:10.1093/toxsci/kfr110
Dai Q, Zhao J, Qi X et al (2014) MicroRNA profiling of rats with ochratoxin A nephrotoxicity. BMC Genomics 15:333. doi:10.1186/1471-2164-15-333
De Santis B, Brera C, Mezzelani A et al (2017a) Role of mycotoxins in the pathobiology of autism: a first evidence. Nutr Neurosci 1–13. doi:10.1080/1028415X.2017.1357793
De Santis B, Raggi ME, Moretti G et al (2017b) Study on the association among mycotoxins and other variables in children with autism. Toxins 29;9(7). pii: E203. doi:10.3390/toxins9070203
Deepmala, Slattery J, Kumar N et al (2015) Clinical trials of N-acetylcysteine in psychiatry and neurology: a systematic review. Neurosci Biobehav Rev 55:294–321. doi:10.1016/j.neubiorev.2015.04.015
Denli M, Perez JF (2010) Ochratoxins in feed, a risk for animal and human health: control strategies. Toxins (Basel) 2(5):1065–1077. doi:10.3390/toxins2051065
Eden S, Hashimshony T, Keshet I et al (1998) DNA methylation models histone acetylation. Nature 394(6696):842
EFSA, European Food Safety Authority (2006) EFSA Opinion of the scientific panel on contaminants in the food chain on a request from the commission related to ochratoxin A (OTA) in food Quest. N° EFSA-Q-2005-154. EFSA J 365(2006):1–56. doi:10.2903/j.efsa.2006.365
Fardmanesh H, Shekari M, Movafagh A et al (2016) Upregulation of the double-stranded RNA binding protein DGCR8 in invasive ductal breast carcinoma. Gene 581(2):146–151. doi:10.1016/j.gene.2016.01.033
Faustman EM, Silbernagel SM, Fenske RA, Burbacher TM, Ponce RA (2000) Mechanisms underlying Children’s susceptibility to environmental toxicants. Environ Health Perspect 108(Suppl 1):13–21
Gayathri L, Dhivya R, Dhanasekaran D et al (2015) Hepatotoxic effect of ochratoxin A and citrinin, alone and in combination, and protective effect of vitamin E: in vitro study in HepG2 cell. Food Chem Toxicol 83:151–163. doi:10.1016/j.fct.2015.06.009
Guo M, Huang K, Chen S, Qi X, He X, Cheng WH, Luo Y, Xia K, Xu W (2014) Combination of metagenomics and culture-based methods to study the interaction between ochratoxin a and gut microbiota. Toxicol Sci 141(1):314–323. doi:10.1093/toxsci/kfu128
Hagelberg S, Hult K, Fuchs R (1989) Toxicokinetics of ochratoxin A in several species and its plasma-binding properties. J Appl Toxicol 9(2):91–96
Hennemeier I, Humpf HU, Gekle M et al (2014) Role of microRNA-29b in the ochratoxin A-induced enhanced collagen formation in human kidney cells. Toxicology 3(324):116–122. doi:10.1016/j.tox.2014.07.012
Hope JH, Hope BE (2012) A review of the diagnosis and treatment of ochratoxin A inhalational exposure associated with human illness and kidney disease including focal segmental glomerulosclerosis. J Environ Public Health 2012:835059. doi:10.1155/2012/835059
Jafari N, Dogaheh HP, Bohlooli S et al. (2013) Expression levels of microRNA machinery components Drosha, Dicer and DGCR8 in human (AGS, HepG2, and KEYSE-30) cancer cell lines. Int J Clin Exp Med 6(4):269–274
Jennings P, Weiland C, Limonciel A et al (2012) Transcriptomic alterations induced by ochratoxin A in rat and human renal proximal tubular in vitro models and comparison to a ratinvivo model. Arch Toxicol 86:571–589
Jennings P, Limonciel A, Felice L, Leonard MO (2013) An overview of transcriptional regulation in response to toxicological insult. Arch Toxicol 87:49–72
Jin J, Cheng Y, Zhang Y et al (2012) Interrogation of brain miRNA and mRNA expression profiles reveals a molecular regulatory network that is perturbed by mutant huntingtin. J Neurochem 123(4):477–490. doi:10.1111/j.1471-4159.2012.07925.x
Kim B, Lee JH, Park JW et al (2014) An essential microRNA maturing microprocessor complex component DGCR8 is up-regulated in colorectal carcinomas. Clin Exp Med 14(3):331–336. doi:10.1007/s10238-013-0243-8
Kriegel AJ, Liu Y, Fang Y et al (2012) The miR-29 family: genomics, cell biology, and relevance to renal and cardiovascular injury. Physiol Genomics 44:237–244
Kumar MS, Lu J, Mercer KL et al (2007) Impaired microRNA processing enhances cellular transformation and tumorigenesis. Nature Genet 39:673–677
Limonciel A, Jennings P (2014) A review of the evidence that ochratoxin A is an Nrf2 inhibitor: implications for nephrotoxicity and renal carcinogenicity. Toxins (Basel) 6(1):371–379. doi:10.3390/toxins6010371
Loboda A, Damulewicz M, Pyza E et al (2016) Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism. Cell Mol Life Sci 73(17):3221–3247. doi:10.1007/s00018-016-2223-0
Lu J, Clark AG (2012) Impact of microRNA regulation on variation in human gene expression. Genome Res 22(7):1243–1254
Lugli G, Torvik VI, Larson J, Smalheiser NR (2008) Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain. J Neurochem 106(2):650–661. doi:10.1111/j.1471-4159.2008.05413.x
Lyu JW, Yuan B, Cheng TL et al (2016) Reciprocal regulation of autism-related genes MeCP2 and PTEN via microRNAs. Sci Rep 6:20392. doi:10.1038/srep20392
MacDonald BT, Tamai K, He X (2009) Wnt/beta-catenin signaling: components, mechanisms, and diseases. Dev Cell 17(1):9–26. doi:10.1016/j.devcel.2009.06.016
Magenta A, Cencioni C, Fasanaro P et al (2011) MC. miR-200c is upregulated by oxidative stress and induces endothelial cell apoptosis and senescence via ZEB1 inhibition. Cell Death Differ 18(10):1628–1639. doi:10.1038/cdd.2011.42
Malir F, Ostry V, Pfohl-Leszkowicz A et al (2016) Ochratoxin A: 50 years of research. Toxins (Basel) 8(7):191. doi:10.3390/toxins8070191
Mally A, Pepe G, Ravoori S et al (2005) Ochratoxin a causes DNA damage and cytogenetic effects but no DNA adducts in rats. Chem Res Toxicol 18(8):1253–1261. PubMed PMID: 16097798
Manners MT, Tian Y, Zhou Z, Ajit SK (2015) MicroRNAs downregulated in neuropathic pain regulate MeCP2 and BDNF related to pain sensitivity. FEBS Open Bio 5:733–740
Marin-Kuan M, Nestler S, Verguet C et al (2007) MAPK-ERK activation in kidney of male rats chronically fed ochratoxin A at a dose causing a significant incidence of renal carcinoma. Toxicol Appl Pharmacol 224(2):174–181
McLaughlin J, Padfield PJ, Burt JP, O’Neill CA (2004) Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms. Am J Physiol Cell Physiol 287(5):C1412–C1417
McMasters DR, Angelo Vedani A (1999) Ochratoxin Binding to Phenylalanyl-tRNA Synthetase:  Computational Approach to the Mechanism of Ochratoxicosis and Its Antagonism. Journal of Medicinal Chemistry 42(16):3075–3086
Mellios N, Sur M (2012) The emerging role of microRNAs in schizophrenia and autism spectrum disorders. Front Psych 3:39. doi:10.3389/fpsyt.2012.00039
Mor F, Kilic MA, Ozmen O et al (2014) The effects of orchidectomy on toxicological responses to dietary ochratoxin A in Wistar rats. Exp Toxicol Pathol 66(5-6):267–275. doi:10.1016/j.etp.2014.04.002
Murer MG, Yan Q, Raisman-Vozari R (2001) Brain-derived neurotrophic factor in the control human brain, and in Alzheimer’s disease and Parkinson’s disease. Prog Neurobiol 63:71–124. doi:10.1016/S0301-0082(00)00014-9
Oba S, Kumano S, Suzuki E et al (2010) miR-200b precursor can ameliorate renal tubulointerstitial fibrosis. PLoS One 5(10):e13614. doi:10.1371/journal.pone.0013614
Pastor L, Vettorazzi A, Campión J, Cordero P, López de Cerain A (2016) Gene expression kinetics of renal transporters induced by ochratoxin A in male and female F344 rats. Food Chem Toxicol 98(Pt B):169–178. doi:10.1016/j.fct.2016.10.019
Preissner SC, Hoffmann MF, Preissner R, Dunkel M, Gewiess A, Preissner S (2013) Polymorphic cytochrome P450 enzymes (CYPs) and their role in personalized therapy. PLoS One 8(12):e82562. doi:10.1371/journal.pone.0082562
Ringot D, Chango A, Schneider YJ, Larondelle Y (2006) Toxicokinetics and toxicodynamics of ochratoxin A, an update. Chem Biol Interact 159(1):18–46
Roshan R, Shridhar S, Sarangdhar MA et al (2014) Brain-specific knockdown of miR-29 results in neuronal cell death and ataxia in mice. RNA 20(8):1287–1297. doi:10.1261/rna.044008.113
San Román MS, Holgado MJ (2015) Intercalation of phenylalanine, isocoumarin and ochratoxin A (OTA) into LDH’s. Open Journal of Inorganic Chemistry 5:52–62. doi:10.4236/ojic.2015.53007
Sand M, Skrygan M, Georgas D, Arenz C, Gambichler T, Sand D, Altmeyer P, Bechara FG (2012) Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer. Mol Carcinog 51(11):916–922. doi:10.1002/mc.20861
Sava V, Reunova O, Velasquez A, Harbison R, Sanchez-Ramos J (2006) (2006a). Acute neurotoxic effects of the fungal metabolite ochratoxin-A. Neurotoxicology 27:82–92. doi:10.1016/j.neuro.2005.07.004
Schilter B, Marin-Kuan M, Delatour T et al (2005) Ochratoxin A: potential epigenetic mechanisms of toxicity and carcinogenicity. Food Addit Contam 22(Suppl 1):88–93
Stachurska A, Ciesla M, Kozakowska M et al (2013) Cross-talk between microRNAs, nuclear factor E2-related factor 2, and heme oxygenase-1 in ochratoxin A-induced toxic effects in renal proximal tubular epithelial cells. Mol Nutr Food Res 57(3):504–515. doi:10.1002/mnfr.201200456
Sun Z, Chin YE, Zhang DD (2009) Acetylation of Nrf2 by p300/CBP augments promoter-specific DNA binding of Nrf2 during the antioxidant response. Mol Cell Biol 29(10):2658–2672. doi:10.1128/MCB.01639-08
Ueta E, Kodama M, Sumino Y et al (2010) Gender-dependent differences in the incidence of ochratoxin A-induced neural tube defects in the Pdn/Pdn mouse. CongenitAnom (Kyoto) 50(1):29–39. doi:10.1111/j.1741-4520.2009.00255.x. PubMed PMID: 20201966
Waxman DJ, Holloway MG (2009) Sex differences in the expression of hepatic drug metabolizing enzymes. Mol Pharmacol 76(2):215–228. doi:10.1124/mol.109.056705
Woodmansey EJ (2007) Intestinal bacteria and ageing. Appl Microbiol 102(5):1178–1186
Wu Q, Dohnal V, Huang L et al (2011) Metabolic pathways of ochratoxin A. Curr Drug Metab 12(1):1–10
Xu J, Zhu X, Wu L et al (2012) MicroRNA-122 suppresses cell proliferation and induces cell apoptosis in hepatocellular carcinoma by directly targeting Wnt/β-catenin pathway. Liver Int 32(5):752–760. doi:10.1111/j.1478-3231.2011.02750.x
Xue ZQ, He ZW, Yu JJ et al (2015) Non-neuronal and neuronal BACE1 elevation in association with angiopathic and leptomeningeal β-amyloid deposition in the human brain. BMC Neurol 15:71. doi:10.1186/s12883-015-0327-z
Zanic-Grubisić T, Zrinski R, Cepelak I, Petrik J, Radić B, Pepeljnjak S (2000) Studies of ochratoxin A-induced inhibition of phenylalanine hydroxylase and its reversal by phenylalanine. Toxicol Appl Pharmacol 167(2):132–139
Zhang X, Boesch-Saadatmandi C, Lou Y, Wolffram S, Huebbe P, Rimbach G (2009) Ochratoxin A induces apoptosis in neuronal cells. Genes Nutr 4(1):41–48. doi:10.1007/s12263-008-0109-y
Zhu L, Yu T, Qi X, Yang B, Shi L, Luo H, He X, Huang K, Xu W (2016) miR-122 plays an important role in ochratoxin A-induced hepatocyte apoptosis in vitro and in vivo. Toxicol Res 5:160–167
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Mezzelani, A. (2017). Ochratoxin A and Epigenetics. In: Patel, V., Preedy, V. (eds) Handbook of Nutrition, Diet, and Epigenetics. Springer, Cham. https://doi.org/10.1007/978-3-319-31143-2_33-1
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