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Targeting Induced Local Lesions in Genomes: A Transgenic Approach for the Improvement of Desirable Crop in the Current Era of the Changing Climate

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Food Production, Diversity, and Safety Under Climate Change

Part of the book series: Advances in Science, Technology & Innovation ((ASTI))

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Abstract

TILLING (Targeting Induced Local Lesions in Genomes) was first shown in 2000 as an anti-hereditary special tool that strengthens traditional mutagenesis and high-throughput screening. In order to take care of the ever-growing population, it is seeking to limit the availability of food with limited land and water resources under a changing climate system. Traditional breeding has played an important role in increasing crop yields but takes long time and hard work. Later, transgenic technology came into being and played an important role in increasing crop yields. Therefore, more emphasis is now needed on crop improvement over non-GM technology. Nowadays, genome editing (GE) technique is also used in crop improvement projects because of its simplicity, robustness and high performance. Therefore, TILLING is a fast, simple, low cost, effective, widely available technique, independent of genotype and genome size. This review focuses on the prospect and potential use of the TILLING technique in crop improvement programs during valid genome editing.

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Abbreviations

AGE:

Agarose Gel Electrophoresis

Az:

Sodium Azide

Az-MNU:

Sodium Azide plus Methylnitrosourea

CE:

Capillary Electrophoresis

DEMETER:

5-Methylcytosine DNA Glycosylase

DEB:

Diepoxybutane

DES:

Diethyl Sulfate

EMS:

Ethyl Methanesulfonate

FN:

Fast Neutron Bombardment

GR:

Gamma Ray Bombardment

HRM:

High-Resolution Melting

PAGE:

Polyacrylamide Gel Electrophoresis

PCR:

Polymerase Chain Reaction

RNAi:

Ribonucleic Acid Interference

TILLING:

Targeting Induced Local Lesions in Genomes

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Authors and Affiliations

  1. Department of Botany, Faculty of Natural Sciences, Shah Abdul Latif University, Khairpur, Mirs Sindh, Pakistan

    Ayaz Latif Siyal & Ali Gohar Chang

  2. Institute of Plant Sciences, University of Sindh Jamshoro, Sindh, Pakistan

    Shumaila Sial

  3. Bangladesh Wheat and Maize Research Institute, Dinajpur, 5200, Bangladesh

    Akbar Hossain

  4. IBA Public School Sukkur, Sindh, Pakistan

    Ayaz Latif Siyal & Ali Gohar Chang

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  1. Ayaz Latif Siyal
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  2. Shumaila Sial
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  3. Akbar Hossain
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Editors and Affiliations

  1. Department of Botany, Acharya Prafulla Chandra Roy Government College, Matigara, Siliguri, West Bengal, India

    Rakhi Chakraborty

  2. Department of Botany, University of North Bengal, Raja Rammohunpur, Siliguri, West Bengal, India

    Piyush Mathur

  3. Department of Botany, University of North Bengal, Raja Rammohunpur, Siliguri, West Bengal, India

    Swarnendu Roy

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Siyal, A.L., Sial, S., Hossain, A., Chang, A.G. (2024). Targeting Induced Local Lesions in Genomes: A Transgenic Approach for the Improvement of Desirable Crop in the Current Era of the Changing Climate. In: Chakraborty, R., Mathur, P., Roy, S. (eds) Food Production, Diversity, and Safety Under Climate Change. Advances in Science, Technology & Innovation. Springer, Cham. https://doi.org/10.1007/978-3-031-51647-4_19

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