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Hydrophilic/Phobic Tailored Multi-laned/Layer Matrix-Assisted Laser Desorption/Ionization (HTML-MALDI)

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Advanced Materials for Multidisciplinary Applications

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Abstract

This study aimed to enhance ion yield from a protein mixture using hydrophobic and hydrophilic structured surfaces to enhance analyte solubilization and the spatial separation of matrices to enable multi-matrix desorption and ionization of the peptide mixtures. The use of solid-stated anchored compounds can aid in sample cleanup and digestion, further improving the signal intensity of digests. However, this research suggested that the results were partly dependent upon the protein's basicity. The more basic lysozyme was found to generate the highest sum peptide intensities under the dried drop methods. On the other hand, the less basic myoglobin produced the highest sum intensities with anchor support. It was critical to use the matrix species in the middle lane for optimizing analyte peak intensities, although using matrices in peripheral lanes did lead to signal enhancements.

It is with profound sadness that SB writes this acknowledgment. There is a word in the Old Testament, salah, meaning to pause and reflect. I deeply miss Peter, but I will never forget the important lessons he taught me about being a scientist, teacher, and human. JL Liu writes I never met Dr. Derrick, but his knowledge to advance science and responsibility to educate the young generation has inspired me. There are three very painful experiences in my life: the death of my grandparents, who raised me and taught me to be a nice person; the death of my parents, who gave me life and encouraged me to be a strong person; and the death of Dr. Derrick who greatly affected Dr. Bashir. I have known and worked with Dr. Bashir for over a decade. I know the type of man he is and know this is largely due to Peter Derrick. I shall never forget the sweet, courageous, and dedicated man whose name will always linger in my thoughts. Thank you, Dr. Peter Derrick, and God Speed.

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References

  1. McEwen CN, Simonsick WJ, Larsen BS, Ute K, Hatada K (1995) The fundamentals of applying electrospray ionization mass spectrometry to low-mass poly (methyl methacrylate) polymers. J Am Soc Mass Spectrom 6(10):906–911

    Article  CAS  Google Scholar 

  2. Nielen MW (1999) MALDI time-of-flight mass spectrometry of synthetic polymers. Mass Spectrom Rev 18(5):309–344

    Article  CAS  Google Scholar 

  3. Beavis RC, Chait BT (1990) High-accuracy molecular mass determination of proteins using matrix-assisted laser desorption mass spectrometry. Anal Chem 62(17):1836–1840

    Article  CAS  Google Scholar 

  4. Hillenkamp F, Karas M (1990) Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization. Methods Enzymol 193:280–295

    Article  CAS  Google Scholar 

  5. Huberty MC, Vath JE, Yu W, Martin SA (1993) Site-specific carbohydrate identification in recombinant proteins using MALD-TOF MS. Anal Chem 65(20):2791–2800

    Article  CAS  Google Scholar 

  6. Harvey DJ (1999) Matrix-assisted laser desorption/ionization mass spectrometry of carbohydrates. Mass Spectrom Rev 18(6):349–450

    Article  CAS  Google Scholar 

  7. Tang K, Allman SL, Chen CH (1992) Mass spectrometry of laser-desorbed oligonucleotides. Rapid Commun Mass Spectrom 6(6):365–368

    Article  CAS  Google Scholar 

  8. Karas M, Hillenkamp F (1988) Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60(20):2299–2301

    Article  CAS  Google Scholar 

  9. Zenobi R, Knochenmuss R (1998) Ion formation in MALDI mass spectrometry. Mass Spectrom Rev 17(5):337–366

    Article  CAS  Google Scholar 

  10. Ehring H, Karas M, Hillenkamp F (1992) Role of photoionization and photochemistry in ionization processes of organic molecules and relevance for matrix-assisted laser desorption ionization mass spectrometry. J Mass Spectrom 27(4):472–480

    CAS  Google Scholar 

  11. Beavis RC, Chaudhary T, Chait BT (1992) α-Cyano-4-hydroxycinnamic acid as a matrix for matrix-assisted laser desorption mass spectrometry. J Mass Spectrom 27(2):156–158

    CAS  Google Scholar 

  12. Gimon ME, Preston LM, Solouki T, White MA, Russell DH (1992) Are proton transfer reactions of excited states involved in UV laser desorption ionization?. J Mass Spectrom 27(7):827–830

    CAS  Google Scholar 

  13. Knochenmuss R (2006) Ion formation mechanisms in UV-MALDI. Analyst 131(9):966–986

    Article  CAS  Google Scholar 

  14. Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yoshida T, Matsuo T (1988) Protein and polymer analyses up to m/z 100 000 by laser ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom 2(8):151–153

    Article  CAS  Google Scholar 

  15. Strupat K, Karas M, Hillenkamp F (1991) 2, 5-Dihydroxybenzoic acid: a new matrix for laser desorption—ionization mass spectrometry. Int J Mass Spect Ion Proces 111:89–102

    Article  CAS  Google Scholar 

  16. Karas M, Ehring H, Nordhoff E, Stahl B, Strupat K, Hillenkamp F, Krebs B et al (1993) Matrix-assisted laser desorption/ionization mass spectrometry with additives to 2, 5-dihydroxybenzoic acid. J Mass Spectrom 28(12):1476–1481

    CAS  Google Scholar 

  17. Karas M, Krüger R (2003) Ion formation in MALDI: the cluster ionization mechanism. Chem Rev 103(2):427–440

    Article  CAS  Google Scholar 

  18. Xu N, Huang ZH, Watson JT, Gage DA (1997) Mercaptobenzothiazoles: a new class of matrices for laser desorption ionization mass spectrometry. J Am Soc Mass Spectrom 8(2):116–124

    Article  CAS  Google Scholar 

  19. Nonami H, Tanaka K, Fukuyama Y, Erra‐Balsells R (1998) β‐Carboline alkaloids as matrices for UV‐matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry in positive and negative ion modes. analysis of proteins of high molecular mass and of cyclic and acyclic oligosaccharides. Rapid Comm Mass Spect 12(6):285–296

    Google Scholar 

  20. Juhasz P, Costello CE (1992) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of underivatized and permethylated gangliosides. J Am Soc Mass Spectrom 3(8):785–796

    Article  CAS  Google Scholar 

  21. Bartlett MG, Busch KL, Wells CA, Schey KL (1996) Use of 2-hydroxy-1-naphthoic acid as a matrix for matrix-assisted laser desorption/ionization mass spectrometry of low molecular weight porphyrins and peptides. J Mass Spectrom 31(3):275–279

    Article  CAS  Google Scholar 

  22. Tang X, Vertes A, Dreifuss PA (1995) New matrices and accelerating voltage effects in matrix-assisted laser desorption/ionization of synthetic polymers. Rapid Commun Mass Spectrom 9(12):1141–1147

    Article  CAS  Google Scholar 

  23. Montaudo G, Montaudo MS, Puglisi C, Samperi F, Daolio S (1994) 2-(4-hydroxyphenylazo)-benzoic acid: A solid matrix for matrix-assisted laser desorption/ionization of polystyrene. Rapid Commun Mass Spectrom 8(12):1011–1015

    Article  CAS  Google Scholar 

  24. Donny Liu HM, Schlunegger UP (1996) Matrix-assisted laser desorption/ionization of synthetic polymers with Azo compound matrices. Rapid Commun Mass Spectrom 10(5):483–489

    Article  Google Scholar 

  25. Jaskolla TW, Lehmann WD, Karas M (2008) 4-Chloro-α-cyano cinnamic acid is an advanced, rationally designed MALDI matrix. Proc Natl Acad Sci 105(34):12200–12205

    Article  CAS  Google Scholar 

  26. Glückmann M, Pfenninger A, Krüger R, Thierolf M, Karasa M, Horneffer V, Strupat K et al (2001) Mechanisms in MALDI analysis: surface interaction or incorporation of analytes? Int J Mass Spectrom 210:121–132

    Article  Google Scholar 

  27. Fournier I, Brunot A, Tabet JC, Bolbach G (2005) Delayed extraction experiments using a repulsing potential before ion extraction: evidence of non‐covalent clusters as ion precursor in UV matrix‐assisted laser desorption/ionization. Part II—Dynamic effects with α‐cyano‐4‐hydroxycinnamic acid matrix. J Mass Spect 40(1):50–59

    Google Scholar 

  28. Bashir S, Derrick PJ, Mutter R (2004) Parameterizing matrix-assisted laser desorption/ionization (MALDI): effect of solvents and co-additives on analyte peak intensities. Eur J Mass Spectrom 10(4):487–493

    Article  CAS  Google Scholar 

  29. Jaskolla TW, Karas M (2011) Compelling evidence for a lucky survivor and gas phase protonation: the unified MALDI analyte protonation mechanism. J Am Soc Mass Spectrom 22(6):976–988

    Article  CAS  Google Scholar 

  30. Giannakopulos AE, Bashir S, Derrick PJ (1998) Comment: Reproducibility of spectra and threshold fluence in matrix-assisted laser desorption/ionization (MALDI) of polymers. Eur Mass Spectrom 4(2):127–131

    Article  CAS  Google Scholar 

  31. Breaux GA, Green-Church KB, France A, Limbach PA (2000) Surfactant-aided, matrix-assisted laser desorption/ionization mass spectrometry of hydrophobic and hydrophilic peptides. Anal Chem 72(6):1169–1174

    Article  CAS  Google Scholar 

  32. Schuerenberg M, Luebbert C, Eickhoff H, Kalkum M, Lehrach H, Nordhoff E (2000) Prestructured MALDI-MS sample supports. Anal Chem 72(15):3436–3442

    Article  CAS  Google Scholar 

  33. Redeby T, Roeraade JE (2004) A simple fabrication of a structured matrix-assisted laser desorption/ionization target coating for increased sensitivity in mass spectrometric analysis of membrane proteins. Rapid Comm in Mass Spect 18(10):1161–1166

    Google Scholar 

  34. Preston LM, Murray KK, Russell DH (1993) Reproducibility and quantization of matrix-assisted laser desorption ionization mass spectrometry: effects of nitrocellulose on peptide ion yields. Biol Mass Spectrom 22(9):544–550

    Article  CAS  Google Scholar 

  35. Brockman AH, Dodd BS, OrlandoRA (1997) desalting approach for MALDI-MS using on-probe hydrophobic self-assembled monolayers. Anal Chem 69(22):4716–4720

    Google Scholar 

  36. Miliotis T, Marko-Varga G, Nilsson J, Laurell T (2001) Development of silicon microstructures and thin-film MALDI target plates for automated proteomics sample identifications. J Neurosci Methods 109(1):41–46

    Article  CAS  Google Scholar 

  37. Stensballe A, Andersen S, Jensen ON (2001) Characterization of phosphoproteins from electrophoretic gels by nanoscale Fe(III) affinity chromatography with off-line mass spectrometry analysis. Proteomics 1(2):207–222

    Article  CAS  Google Scholar 

  38. Xu S, Li Y, Zou H, Qiu J, Guo Z, Guo B (2003) Carbon nanotubes as an assisted matrix for laser desorption/ionization time-of-flight mass spectrometry. Anal Chem 75(22):6191–6195

    Article  CAS  Google Scholar 

  39. Leize EM, Leize EJ, Leize MC, Voegel JC, Dorsselaer A (1999) Matrix-assisted laser desorption ionization mass spectrometry: a new tool for probing interactions between proteins and metal surfaces. Use in dental implantology. Anal Biochem 272(1):19–25

    Article  CAS  Google Scholar 

  40. Teng CH, Ho KC, Lin YS, Chen YC (2004) Gold nanoparticles as selective and concentrating probes for samples in MALDI MS analysis. Anal Chem 76(15):4337–4342

    Article  CAS  Google Scholar 

  41. Hilton A, Armstrong RA (2006) Statnote 6: post-hoc ANOVA tests. Microbiol, 34–36. https://publications.aston.ac.uk/id/eprint/39919/

  42. Tauer K, Ali AI, Yildiz U, Sedlak M (2005) On the role of hydrophilicity and hydrophobicity in aqueous heterophase polymerization. Polymer 46(4):1003–1015

    Article  CAS  Google Scholar 

  43. Save M, Weaver JVM, Armes SP, McKenna P (2002) Atom transfer radical polymerization of hydroxy-functional methacrylates at ambient temperature: comparison of glycerol monomethacrylate with 2-hydroxypropyl methacrylate. Macromolecules 35(4):1152–1159

    Article  CAS  Google Scholar 

  44. Haddleton DM, Waterson C, Derrick PJ, Jasieczek CB, Shooter AJ (1997) Monohydroxy terminally functionalized poly (methyl methacrylate) from atom transfer radical polymerization. Chem Commun 7:683–684

    Article  Google Scholar 

  45. Jacobs A, Dahlman O (2001) Enhancement of the quality of MALDI mass spectra of highly acidic oligosaccharides by using a Nafion-coated probe. Anal Chem 73(3):405–410

    Article  CAS  Google Scholar 

  46. Landry F, Lombardo CR, Smith JW (2000) A method for application of samples to matrix-assisted laser desorption ionization time-of-flight targets that enhances peptide detection. Anal Biochem 279(1):1–8

    Article  CAS  Google Scholar 

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Acknowledgments

SB would like to thank Drs. I. Herbert and J. Horsler (Accordis, U.K) for access to the Bruker Biflex. The Department of Physics for access to the scanning electron microscope (Warwick University), and Dr. A.E. Giannakopulos (Warwick University) for their helpful suggestions concerning the manuscript. SB would also like to acknowledge financial support from the Engineering and Physical Sciences Research Council (EPSRC), Akzo Noble, and the Robert A. Welch Foundation Departmental Grant (AC006). LL would like to thank the Petroleum Research Fund of the American Chemical Society (53,827-UR10).

A photograph of Doctor Peter Derrick.

Author information

Authors and Affiliations

  1. Institute of Mass Spectrometry, University of Warwick, Coventry, CV4 7AL, UK

    Sajid Bashir & Peter J. Derrick

  2. Department of Chemistry, Texas A&M University-Kingsville, 700 University Blvd., Kingsville, TX, USA

    Sajid Bashir & Jingbo Liu

  3. Department of Physics, The University of Auckland, 7 Symonds Street, Auckland, 1061, New Zealand

    Peter J. Derrick

Authors
  1. Sajid Bashir
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  2. Jingbo Liu
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  3. Peter J. Derrick
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Contributions

Dr. Peter Derrick conceived the research idea and access to the laboratory and supplies. SB undertook all the experimental research, JL verified the data and suggested the ‘temperature at surface’ desorption mechanism.

Corresponding author

Correspondence to Sajid Bashir .

Editor information

Editors and Affiliations

  1. Chinese American Chemical Society, Castro Valley, CA, USA

    Marinda Wu

  2. Corporate Research and Development, The Dow Chemical Company, Collegeville, PA, USA

    Wei Gao

  3. Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA

    Lei Li

  4. Chinese American Chemical Society, South Brunswick, NJ, USA

    Yingchun Lu

  5. Department of Chemistry, Texas A&M University—Kingsville, Kingsville, TX, USA

    Jingbo Louise Liu

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The authors have no conflict of interest with the editorial staff, publishers, or funding agencies supporting this research.

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Bashir, S., Liu, J., Derrick, P.J. (2024). Hydrophilic/Phobic Tailored Multi-laned/Layer Matrix-Assisted Laser Desorption/Ionization (HTML-MALDI). In: Wu, M., Gao, W., Li, L., Lu, Y., Liu, J.L. (eds) Advanced Materials for Multidisciplinary Applications. Springer, Cham. https://doi.org/10.1007/978-3-031-39404-1_13

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