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NADPH Oxidases in Zebrafish

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NADPH Oxidases Revisited: From Function to Structure

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Abstract

Zebrafish, a minnow family teleost fish, has emerged as a powerful in vivo vertebrate model system in biological research. Zebrafish have been used to investigate a number of biological functions mediated by NADPH oxidase (Nox), a transmembrane enzyme known for its role in the production of reactive oxygen species (ROS). Several Nox isoforms such as Nox1, Nox2, Nox4, Nox5, and Duox are expressed in zebrafish. Nox expression and activity change dynamically during zebrafish development and play a role in cell signaling, embryo development, physiology, and pathophysiology. A classical role of Nox is infection control mediated by macrophages and leukocytes, which produce ROS during the oxidative burst. Nox-derived hydrogen peroxide (H2O2) after injury acts as a critical signaling molecule and mediates migration of leukocytes during wound response. Nox also plays a role in several other aspects of zebrafish development and physiology including nervous system development, sensory axon regeneration, thyroid hormone synthesis, and circadian clock control. In addition, zebrafish are used as a model to study Nox function in human disease such as congenital hypothyroidism. Because of its many advantages, zebrafish will likely continue serving as an excellent model system for investigating Nox functions in various biological processes.

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Acknowledgements

Work in the Suter Lab is supported by NIH grant R01NS117701 and a grant from the Indiana Spinal Cord and Brain Injury Research Fund.

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  1. Department of Biological Sciences, Purdue Institute for Integrative Neuroscience, Purdue University, West Lafayette, IN, USA

    S. M. Sabbir Alam & Daniel M. Suter

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  1. S. M. Sabbir Alam
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Correspondence to Daniel M. Suter .

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  1. Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

    Edgar Pick

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Alam, S.M.S., Suter, D.M. (2023). NADPH Oxidases in Zebrafish. In: Pick, E. (eds) NADPH Oxidases Revisited: From Function to Structure. Springer, Cham. https://doi.org/10.1007/978-3-031-23752-2_29

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