Abstract
This study employed taurine as a feed additive to explore the prophylactic effect of taurine on LPS-induced hepatic injury in piglets. The pathological shifts within hepatic tissue were observed by HE staining. Serum levels of ALT and AST together with SOD, CAT, GSH-PX activity, and MDA serum and liver levels were detected. TUNEL was used to detect apoptosis, while qPCR was employed to detect HO-1, Nrf-2, Bcl2, BAX, Caspase-3, and NF- κB p65 transcriptomic expression levels. TRL4, Caspase-3, Nrf-2, and NF- κB p-p65/NF- κB p65 were detected by Western blot. The results revealed that taurine reduces LPS-induced pathological damage of hepatic tissue and reduces the levels of ALT and AST in pig serum. The transcriptomic expression levels of HO-1 and Nrf-2 were upregulated, and proteomic expression of Nrf-2 was increased. SOD, CAT, and GSH-PX activity was elevated, while MDA content was reduced in serum and liver. The levels of mRNA of BAX and Caspase-3 were downregulated, but mRNA content of Bcl2 was increased, and the protein levels of TRL4, NF-κB p-p65/NF-κB p65, and Caspase-3 were diminished. Overall, the degree of hepatocyte apoptosis was also significantly reduced. In conclusion, taurine reduces LPS-induced injury of piglet liver, while reducing hepatocyte apoptotic levels. These data provide a scientific basis for the selection of animal feed additives and lay a foundation for the healthy and sustainable development of the porcine industry.
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Abbreviations
- LPS:
-
lipopolysaccharide
- OS:
-
oxidative stress
- AST:
-
aspartate; aminotransferase
- ROS:
-
reactive oxygen species
- ALT:
-
glutamic-pyruvic transaminase
- CAT:
-
catalase
- SOD:
-
superoxide dismutase
- HO-1:
-
heme oxygenase-1
- Nrf-2:
-
nuclear factor-E2-related factor2
- MDA:
-
malondialdehyde
- MAPK:
-
mitogen activated protein kinase
- Bcl-2:
-
B-cell lymphoma-2
- TLR4:
-
toll-like receptors 4
- caspase-3:
-
cysteinyl aspartate specific proteinase
- ARE:
-
antioxidant response element
- GSH-Px:
-
glutathione peroxidase
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This work was supported by the National Natural Science Foundation of China (31872440, 31872441).
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Zhao, Dd. et al. (2022). Taurine Prevents LPS-Induced Liver Injury in Weaned Piglets. In: Schaffer, S.W., El Idrissi, A., Murakami, S. (eds) Taurine 12. Advances in Experimental Medicine and Biology, vol 1370. Springer, Cham. https://doi.org/10.1007/978-3-030-93337-1_45
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