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Simulation of Bacterial Motion Under Flow Inside Micro Channel Using CFD and DPM

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Advances in Manufacturing Processes, Intelligent Methods and Systems in Production Engineering (GCMM 2021)

Part of the book series: Lecture Notes in Networks and Systems ((LNNS,volume 335))

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Abstract

Discrete Phase Method (DPM) is coupled in this study with Computational Fluid Dynamics (CFD) to simulate motion of rod-shaped motile bacteria under flow conditions inside a microfluidic device where a micro scale chamber is connected to same scale inlet and outlet channels. Here, bacterial cells are represented by spherical solid particles with equivalent volume of a typical bacterial cell, and physio-chemical interactions between cells and solid surface are omitted. Bacterial suspension is assumed as a Newtonian fluid in a laminar flow. Particle injection mass flowrate was selected to emulate OD600 0.1 bacterial cell concentration and lowered up to 10% of initial concentration. Particle diffusion pattern was affected by the fluid velocity (within the range 0.05–0.005 m.s−1), but the pattern remined similar for particle mass flowrates varying from 8–80 × 10–9 kg.s−1. Particle mass concentration long the flow direction was varied with fluid velocity but not affected by the injection concentration. At 0.005 m.s−1 fluid velocity, maximum particle mass concentration varied with a linear relationship with particle injection mass flowrate, and the variation at 0.05 m.s−1 fluid velocity too showed a linear relationship with injection mass flowrate but with higher gradient. This is a new finding on P. aeruginosa cell motion and diffusion inside microchannels.

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Acknowledgements

Authors wishes to acknowledge support from, Institute of Health and Biomedical Innovation (IHBI), Faculty of Engineering, Queensland University of Technology (QUT), Australia, Advance Queensland Industry Research Fellowship, Konica Minolta and Bionics Queensland. The first author is a lecturer from University of Moratuwa, Sri Lanka, currently attached to QUT. Funding was received for this work from AHEAD project (Grant: AHEAD/PhD/R2/ENG/TECH/161), University Grants Commission of Sri Lanka.

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Authors and Affiliations

  1. Faculty of Engineering, Queensland University of Technology, Brisbane, QLD, 4000, Australia

    S. W. M. A. I. Senevirathne, J. Hasan, A. Mathew, M. Woodruff & P. K. D. V. Yarlagadda

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  1. S. W. M. A. I. Senevirathne
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  2. J. Hasan
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  3. A. Mathew
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Correspondence to P. K. D. V. Yarlagadda .

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Editors and Affiliations

  1. Liverpool John Moores University, Liverpool, UK

    Andre Batako

  2. Wroclaw University of Science and Technology, Wrocław, Poland

    Anna Burduk

  3. School of Civil Engineering and Built Environment, Liverpool John Moores University, Liverpool, UK

    Kanisius Karyono

  4. General Engineering Research Institute, Liverpool John Moores University, Liverpool, UK

    Xun Chen

  5. Faculty of Mechanical Engineering, Silesian University of Technology, Gliwice, Poland

    Ryszard Wyczółkowski

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Senevirathne, S.W.M.A.I., Hasan, J., Mathew, A., Woodruff, M., Yarlagadda, P.K.D.V. (2022). Simulation of Bacterial Motion Under Flow Inside Micro Channel Using CFD and DPM. In: Batako, A., Burduk, A., Karyono, K., Chen, X., Wyczółkowski, R. (eds) Advances in Manufacturing Processes, Intelligent Methods and Systems in Production Engineering. GCMM 2021. Lecture Notes in Networks and Systems, vol 335. Springer, Cham. https://doi.org/10.1007/978-3-030-90532-3_8

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