Abstract
The aim of this research is to customize the porosity of biofabricated chitosan membrane (CM) employing co-assembled polystyrene nanoparticles (np) as a sacrificial template. CM with np (CM-np) was manufactured inside the microfluidic chip using the flow-assembly technique. Glutaraldehyde was used to crosslink the fabricated CM-np were then dissolved with dimethyl sulfoxide, leaving the porous chitosan membrane (pCM). The growth rate of CM and CM-np was investigated to determine the effects of np incorporation on the growth of the fabricated membrane. The morphology of the biofabricated CM and pCM were evaluated using scanning electron microscopy. The mass transport tests were also conducted to confirm the increase in pores size of pCM in comparison with pure CM. Thus, in this study, we have demonstrated the capability to manipulate the porosity of the biofabricared CM manufactured by flows inside microfluidic chips and characterized the properties of the fabricated membranes. This tuning process is promising and can enhance the applicability of biopolymer CM in biochemistry and biology researches.
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Acknowledgements
This effort was supported in part by the National Science Foundation (NSF) under grant number CAREER 1553330 and the National Institute of Health (NIH) under grant number 1R15GM129766-01. We would like to acknowledge the support of the Maryland NanoCenter and its AIMLab.
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The authors report no conflicts of interest in this work.
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Ly, K.L., Luo, X. (2022). Fabrication and Characterization of Porous Flow-Assembled Chitosan Membranes in Microfluidics. In: Van Toi, V., Nguyen, TH., Long, V.B., Huong, H.T.T. (eds) 8th International Conference on the Development of Biomedical Engineering in Vietnam. BME 2020. IFMBE Proceedings, vol 85. Springer, Cham. https://doi.org/10.1007/978-3-030-75506-5_31
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