Abstract
Background: Standard semen evaluation according to WHO thresholds (2010) is still insufficient to disclose individuals with a diagnosis of idiopathic infertility. Sperm DNA fragmentation is considered a possible valuable marker, and flow-cytometry-based Sperm Chromatin Structure Assay (SCSA) analysis has been used. However, the method is cumbersome and time-consuming; hardly applicable in a clinical setting where split ejaculates are used for diagnostics and intervention. The present study explores an alternative method using conventional bright-field microscopy (HalospermG2, Halothech, Madrid, Spain) with Sperm DNA fragmentation (SDF) as end values. Analyses were done on split ejaculates (F1, sperm-rich fraction, mainly prostate-dominated versus F2, sperm-poor fraction, mainly seminal vesicle-dominated) of documented fertile donors and partners in a couple seeking for ART-intervention with a clinical diagnosis of idiopathic infertility (patients).
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Reference
Alvarez-Rodriguez M, Liffner S, Vicente-Carrillo A, Hammar M, Rodriguez-Martinez H (2016) Sperm chromatin integrity (SCSA analysis) in specific ejaculate fractions can improve semen evaluation under clinical settings. Annual meeting ESHRE, Helsinki, Finland, 2016. Human Reproduction 31 S1 i163-i164
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Alvarez-Rodríguez, M., Pehrson, I., Liffner, S., Hammar, M., Rodríguez-Martinez, H. (2021). Sperm DNA Fragmentation in Human Split Ejaculates. In: Björndahl, L., Flanagan, J., Holmberg, R., Kvist, U. (eds) XIIIth International Symposium on Spermatology. Springer, Cham. https://doi.org/10.1007/978-3-030-66292-9_12
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DOI: https://doi.org/10.1007/978-3-030-66292-9_12
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