Dichrostachys cinerea Growth Rings as Natural Archives for Climatic Variation in Namibia

Global Circulation Models (GCMs) are used to forecast climate change in Southern Africa, and the evidence shows that the region is going to warm up by up to 2° by the year 2050. Namibia is one of the driest countries in Southern Africa and is at a high risk of becoming much drier than current situation by 57%. Very few studies have been carried out in Southern Africa to show actual impacts of climate change. Practical applicability of GCMs at a local spatial scale remains limited due to the coarse nature of the models. Hence, improvement of the GCMs must begin with better understanding of the local microclimates and how they respond to regional circulation patterns. In many regions of Southern Africa, the lack of potential tools to access old climatic records precludes the estimation of climate trends beyond 100 years. In spite of these impediments, there are areas with excellent tree species such as Dichrostachys cinerea that are able to be used as climatic archives for specific time periods. In this chapter, the study shows that the combination of tree ring chronologies and precipitation records is a powerful methodology in climate modeling in the southern hemisphere and reveals nuances that show climate change. The evaluation of data from tree rings coupled with precipitation trends reveals signals that show that climate has indeed been changing over the past ten decades and will have a negative impact on livelihoods. These data can now be used in predictive models that can be used to project future scenarios and assist policy makers and planners to see how climate will evolve in the next 50–60 years.


Introduction
Current scientific, observational, and anecdotal data show that climate change is now a well-observed phenomenon in most parts of the world (IPCC 2007(IPCC , 2015Shikangalah and Mapani 2019). Traditional livelihoods of subsistence farming, food security, water security, and cultural organizational patterns are likely to be, and some are already impacted by effects of Climate change. Thus, climate change will be a major problem in the near future, impacting water resources, agricultural and food systems in Southern Africa, particularly Namibia (Adhikari et al. 2015). In Namibia, there is an increasing trend observed in flooding, droughts, and evapotranspiration (Zeidler et al. 2010). The shortening of the crop growing season, rising average summer and winter temperatures, is among some of the evidence of climate change impacts in Namibia (Mapaure 2016). Temperature in Namibia has been increasing at three times the global mean, and the increase in temperature prediction for 2100 for Namibia ranges from 2°C to 6°C, particularly in the central regions (Reid et al. 2007).
These impacts will with time increasingly limit water supply, development activities, and consequently will affect all cross-sections of society (Ohlsson and Turton 1999;Showers 2002;Gumbo 2003;Wang et al. 2009). A number of authors have predicted that a quarter of the world's population will experience severe water scarcity within the first quarter of the next century and approximately a billion people in arid regions will face absolute water scarcity by 2025 (Seckler et al. 1999;Ruth et al. 2007). Water demand in urban areas is constantly on the upswing in Africa, Namibia, included (Mapani 2005;Shikangalah and Mapani 2019). The water demand is anticipated to rise by 50% between 2010 and 2030 (Lafforgue 2016).
Trees are excellent archives of environmental site conditions that affect their biological processes (Breitenmoser et al. 2014) and this information is reflected in their growth ring histories. Growth rings provide information about the history of the area in which the trees are resident, including other information such as tree age, fire events, droughts, attacks by pests, logging, and severe storms (Xing et al. 2012;Feliksik and Wilczyński 2009). Growth rings in trees are influenced by climate conditions and they show distinct episodes of moist and dry periods for over time (Novak et al. 2013;Palmer et al. 2018;Shikangalah et al. 2020). Dendrochronology can be used to reconstruct and draw conclusions about rainfall patterns over regional, hemispheric, or even global scales (Zhang 2015;NASA 2008). Tree growth rings are a valuable proxy for climate change studies on both local and regional scales (Nock et al. 2016;Novak et al. 2013;Steenkamp et al. 2008). Data from growth rings records is valuable for providing information that may not be available in areas where climate records never existed in the past. Hence, they aid the understanding of ecosystem dynamics and to predict changes in the ecological surroundings. This information is especially important in arid and semi-arid environments such as Namibia, where sound land management is crucial due to the climate-related vulnerability of such environments and the frequent lack of observational data. This holds particularly true for Namibia as the country is the most arid in Southern Africa and second only in aridity to the Sahara Desert in North Africa (Food and Agriculture Organization (FAO) 2005; Turpie et al. 2010). More than 90% of Namibia's landmass is classified as semi-arid, arid, or hyper-arid, and the country is characterized by sporadic rainfall and high evaporation rates (Mendelsohn et al. 2002;Shanyengana et al. 2004;Barnard 2012). This chapter is aimed at assessing the responsiveness of D. cinerea to different climatic environments and thus be used as a proxy for climate change.

Evidence for Climatic Variation from Dendrochronological Studies
In this study, we selected sites that had a degree of variation in both precipitation and temperature. This climatic gradient revealed how the species responded to different amounts of rainfall and temperatures. The study sites are located at Lake Otjikoto (19°11 0 41 00 S; 17°32 0 59 00 E); Farm Onyoka located on the Waterberg Plateau Park (20°25 0 0 00 S; 17°13 0 0 00 E), and Farm Kuzikus (23°20 0 0 00 S; 18°21 0 59 00 E) located south of Windhoek (Fig. 1). The annual precipitation for the study sites is 550 mm to 600 mm for Lake Otjikoto, 450-500 mm for Onyoka, and less than 300 mm for Kuzikus. These sites also vary in temperature as follows: an average annual temperature of 25°C for Lake Otjikoto, 30°C for Waterberg Plateau Park, and 31°C for Kuzikus (Mendelsohn et al. 2002). Lake Otjikoto has better soils of loamy to clay with a good water holding capacity as opposed to the deep sandy Kalahari soils (Kříbek et al. 2018;Mendelsohn et al. 2002;Rodgers et al. 2017).

Samples and Data Processing
The precipitation data for 1970-2014 were obtained from Namibian official Meteorological Service in Windhoek. The growth rings of Dichrostachys cinerea were used, a native species to Namibia which is a fast-growing species that has become an undesirable encroacher and is particularly a problem in areas where there has been overgrazing (Orwa et al. 2009;Chepape et al. 2011;O'Connor et al. 2014). The population of D. cinerea decreases significantly from Lake Otjikoto site where the trees are abundant, to Kuzikus where the trees are very few (Fig. 1).
Wood samples of Dichrostachys cinerea were collected from randomly selected trees from three sites namely Lake Otjikoto, Waterberg, and Kuzikus ( Fig. 1), with a total of 32 samples of D. cinerea. For each tree, one sample disc was taken at a height of about 1.0 m and air dried. The rings were identified using a combination of techniques for correction and validation of ring growth, that is, binocular microscope; WinDENDRO software, which automatically counts and dates each marginal parenchyma; and COFECHA program (Holmes 1983), which is commonly used in dendrochronology to ensure quality and measurement accuracy in growth ring segments Fig. 1 The three study sites Lake Otjikoto (LO), Waterberg (WB), and Kuzikus (KZ) are shown along the rainfall gradient in Namibia (Grissino-Mayer 2001). To make sure that all the rings were correctly detected and dated by WinDENDRO software, at least four different paths radiating from the pith were created and analyzed (Grudd 2006;Heinrich et al. 2009;Shikangalah et al. 2020).
To explore the influence of temporal climatic variability on growth rings, the use of response and correlation functions from tree-ring chronologies and monthly climatic data was employed after the methods of Zang and Biondi (2013). Using the dplR package (Bunn 2008) of R 3.4.4 (R Core Team 2018), the dcc function in TreeClim Package in R was used. The dcc function builds upon and extends the functionality of program DENDROCLIM2002 (Biondi and Waikul 2004;Biondi 2013, 2015). A total of 32 samples were subject to spline detrending (50% frequency) and then averaged to a chronology using Tukey's biweight robust mean. The timeframe used is only for the wet period (November of previous year-April of current year) is used between 1977 and 2015.

Precipitation as Reflected in Tree Rings
Rainfall in Namibia is unequally distributed, even in years when the country receives good rains, not all areas experience the same precipitation levels. Figure 2 shows the annual rainfall at the three study sites for the years 1970-2014. The rainfall distribution at the three sites follows the national trend where precipitation decreases from north-east to south west (Fig. 1). Figure 2 shows rainfall variability over four decades at the three study sites. At Lake Otjikoto, the annual rainfall was above the average (558 mm) for the years 1972-1974, 1976-1978, 1997, 2001, 2006, 2007-2008, and 2011-2012. At Waterberg, the following seasons had above average (471 mm) precipitation, 1973-1974, 1983-1984, 1987-1988, 2001-2001, and 2013-2014 seasons. Kuzikus has the least number of years with above average rainfall (281 mm), namely, 1973-1974, 1997, and 2006-2007. In decadal terms, the decade 1970-1979 had more rainfall than the 1980-1989 at Otjikoto, whereas the decade 1990-1999 had less rainfall than the 1970-1979 but higher rainfall than the   Fig. 3a, the growth of D. cinerea at Kuzikus (annual rainfall of 250-300 mm/a) shows a positive correlation with rainfall except in years when flooding occurred. At Waterberg (Fig. 3b) and Lake Otjikoto (Fig. 3c), the correlation is also positive. In  Figure 4 summarizes the comparisons in ring width growth across the precipitation gradient. Dichrostachys cinerea ring width growth correlates with the amount of precipitation, though the median ring widths did not significantly differ among sites.

Growth Ring Responses to Climatic Variation
Using bootstrapped correlation function analysis (R Core Team 2018), the darker bars in Fig. 5 indicate a coefficient significant at p < 0.05, and the lines represent the 95% confidence interval. Figure 5 shows that the response coefficients of temperature, precipitation, and aridity at Lake Otjikoto and Kuzikus sites. The results shows that at Lake Otjikoto, the temperature, moisture availability, and overall aridity have positive but weak influence on growth rings in some months, mainly November and December (temperature), February (temperature and rainfall), and all three variables during March and April months (Fig. 5a). On contrary at Kuzikus, the influence of moisture availability is still positive but to a lesser extent than at Lake Otjikoto (Fig.  5b). The temperature has a significant negative influence in growth rings in December (darker bar, Response Coefficients (r) ¼ À 0.13). Figure 6 shows the autocorrelation lags of growth ring width with precipitation soil moisture. For Kuzikus, D. cinerea in the period 2000-2009 shows a negative correlation, whereas at Waterberg shows an intermediate response to precipitation soil moisture with growth ring width. The growth ring soil-moisture correlations at Kuzikus where temperatures are high are much weaker compared to the correlations at Lake Otjikoto where rainfall is much higher. Lake Otjikoto shows a very strong positive correlation, way above the normality curve.

Lessons Learnt from Arid and Semi-arid Savannas
Evidence from precipitation (Fig. 2) and tree growth rings has shown that D. cinerea is sympathetic to precipitation soil moisture (Figs. 3, 4, and 5). This relationship is cardinal in the use of tree ring chronologies as proxies to climate variability. Trees are good archives of biomass systematic addition due to their physiological response to water stress that affects growth rings (McCarrol and Loader 2004). Additionally the stomatal conductance is controlled by the relative availability of edaphic Fig. 5 (a) Lake Otjikoto site: D. cinerea growth ring responsiveness to temperature, precipitation, and aridity (climatic factors affecting tree growth the most). (b) Kuzikus site: D. cinerea growth ring responsiveness to temperature, precipitation, and aridity (climatic factors affecting tree growth the most) moisture (precipitation) and external leaf water vapor deficit (humidity) (Woodborne et al. 2015). In Namibia, the wet (rain) season used to start at the beginning of November four decades ago; and there has been a slow but gradual and systematic shift to mid-December in the past decade (Shikangalah and Mapani 2019). Such information is available in precipitation and tree ring growth records (Shikangalah et  al. 2020). Work in Southern Africa has now proven beyond doubt that dendrochronology works as good as in the northern hemisphere for certain species of trees (Gourlay 1995;February 2000;Fichtler et al. 2004;Trouet et al. 2006;Woodborne et al. 2015;Shikangalah et al. 2020). Woodborne et al. (2015) reconstructed a 1000 year carbon isotope rainfall proxy record from the South African Adansonia digitata.
The key significance of soil moisture supply for the tree growth and its apparent influence is indicated in Fig. 4, where the Lake Otjikoto site with a higher mean annual precipitation shows a much higher ring width growth than at the other two sites. The data illustrate that D. cinerea growing in areas with high amounts of rainfall grows wider rings when all other climatic factors are taken together (Fig. 4). The patterns observed in rainfall (Fig. 2) are reflected in the autocorrelation diagrams ( Fig. 6) which is a reflection of the microclimate effects on tree growth ring at each particular location. This suggests that D. cinerea growth rings can be used as a proxy for climatic variations wherever it is present. This finding is echoed by Kwak et al. (2016) who showed that soil moisture or groundwater near flowing streams influenced tree ring width growth and the size of the rings was proportional to the amount of moisture received.
The results show that in the years when floods occurred (Fig. 3), tree ring width did not correspond to the amount of rainfall received. This fact was attributed to runoff effects, where certain species do not add significant biomass under flooded conditions (e.g., Schulman 1945;Astrade and Bégin 1997;Kwak et al. 2016). Thus, there is an optimum maximum soil moisture threshold for D. cinerea.
Southern African species have been for a long time considered as not good at adding biomass systematically on an annual basis. However, tree chronologies for D. cinerea and S. mellifera were established by Shikangalah et al. (2020). Watkins et al. (2018) showed that tree ring sensitivity to climatic variables is dominated by precipitation. It is abundantly clear that for Southern Africa, Namibia, in particular, tree ring studies are excellent proxies for climate variability studies as they act as natural archives that record site conditions and precipitation effects.
The responsiveness of D. cinerea to precipitation, air temperature, and the overall aridity was also examined (Fig. 5). Overall, the response coefficients suggest contrast response of growth rings to climatic conditions at the two sites, with exception of rainfall and aridity in January and February. The low responses are mostly experienced at Kuzikus, while most of the positive responses are experienced at Lake Otjikoto. The response function also showed a precipitation response to a less significant correlation during February to April when the temperatures are cooler after some rainfall. The response coefficients are indicative periods on which the growth rings are quite small, suggesting that D. cinerea trees have a very narrow range of responsiveness in which they can grow, therefore supporting the evidence that they add the least amount of biomass in areas of less rainfall such as at Farm Kuzikus (Fig. 4). Due to the lack of darker bars which show a high significant response, these results demonstrate that growth rings are also influenced by other variables such as type of soil, groundwater, and vegetation.

Conclusions
Growth rings in D. cinerea correlate to the amount of precipitation uptake by the species. Dichrostachys cinerea is dependent on rainfall for its survival, it has colonized wetter sites (Lake Otjikoto and Waterberg) more than it does in drier sites (e.g., Kuzikus). Therefore, it is much more important for farmers in high rainfall areas to anticipate more encroachment by D. cinerea and take necessary interventions to control or manage it. With regard to the usefulness of the species as proxy for modeling climatic conditions, it is concluded that D. cinerea is more responsive to climatic variables as it has proportionally responded to the amount of rainfall with ring growth and temperature. At Lake Otjikoto, growth rings are enhanced by both precipitation and temperature, whereas at Kuzikus, only the precipitation improves growth rings, the temperature effects the growth rings negatively. The findings from this chapter suggest that D. cinerea can be used as a proxy for precipitation shifts with time over relatively short periods, for instance, of 30-to 50-year histories, and where older trees can be found, it can be used to reconstruct climatic changes over several hundred years. Open Access This chapter is licensed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.
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