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Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spectrometry

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Functional Analysis of DNA and Chromatin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1094))

Abstract

Chromatin is unevenly distributed within the eukaryote nucleus and it contributes to the formation of morphologically and functionally distinct substructures, called chromatin domains and nuclear bodies. Here we describe an approach to assess specific chromatin features, the histone posttranslational modifications (PTMs), of the largest nuclear sub-compartment, the nucleolus. In this chapter, methods for the isolation of nucleolus-associated chromatin from native or formaldehyde-fixed cells and the effect of experimental procedures on the outcome of mass spectrometry analysis of histone PTMs are compared.

Stefan Dillinger and Ana Villar Garea have contributed equally to this work.

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Acknowledgments

This work was supported by the Deutsche Forschungsgemeinschaft SFB960 grant.

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Authors and Affiliations

  1. Biochemistry Center Regensburg, University of Regensburg, Regensburg, Germany

    Stefan Dillinger, Ana Villar Garea, Rainer Deutzmann & Attila Németh

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  1. Stefan Dillinger
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  2. Ana Villar Garea
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  3. Rainer Deutzmann
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  4. Attila Németh
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Editor information

Editors and Affiliations

  1. Department of Biology, Autonomous University of Madrid Faculty of Sciences, Madrid, Spain

    Juan C. Stockert

  2. Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Madrid, Spain

    Jesús Espada

  3. Department of Biology, Autonomous University of Madrid Faculty of Sciences, Madrid, Spain

    Alfonso Blázquez-Castro

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Dillinger, S., Garea, A.V., Deutzmann, R., Németh, A. (2014). Analysis of Histone Posttranslational Modifications from Nucleolus-Associated Chromatin by Mass Spectrometry. In: Stockert, J., Espada, J., Blázquez-Castro, A. (eds) Functional Analysis of DNA and Chromatin. Methods in Molecular Biology, vol 1094. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-706-8_22

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  • DOI: https://doi.org/10.1007/978-1-62703-706-8_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-705-1

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