Abstract
The methyl-combing technique combines the dynamic molecular combing method with the detection of DNA modifications. The assay allows the single-molecule analysis of epigenetic marks on regularly stretched DNA fibers, at the megabase scale with kilobase resolution. The protocol presented in this chapter is based on proof-of-principle experiments where the single-molecule detection of DNA methylation has been performed on unmethylated and in vitro methylated λ phage DNA.
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Acknowledgments
The author would like to thank Aaron Bensimon, Thomas Cremer, Gernot Längst, and Michael Rehli for providing reagents and devices.
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Németh, A. (2014). Methyl-Combing: Single-Molecule Analysis of DNA Methylation on Stretched DNA Fibers. In: Stockert, J., Espada, J., Blázquez-Castro, A. (eds) Functional Analysis of DNA and Chromatin. Methods in Molecular Biology, vol 1094. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-706-8_18
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DOI: https://doi.org/10.1007/978-1-62703-706-8_18
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-705-1
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