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Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data

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Mass Spectrometry Data Analysis in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1007))

Abstract

A major component of a hydrogen exchange mass spectrometry experiment is the analysis of protein and peptide mass spectra to yield information about deuterium incorporation. The processing of data that are produced includes the identification of each peptic peptide to create a master table/array of peptide sequence, retention time and retention time range, mass range, and undeuterated mass. The amount of deuterium incorporated into each of the peptides in this array must then be determined. Various software platforms have been developed in order to perform this specific type of data analysis. We describe the fundamental parameters to be considered at each step along the way and how data processing, either by an individual or by software, must approach the analysis.

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Acknowledgments

The authors’ work with HX-MS is supported by the NIH (R01-GM086507) and the Waters Corporation.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA, USA

    Thomas E. Wales & John R. Engen

  2. Waters Corporation, Milford, MA, USA

    Michael J. Eggertson

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  1. Thomas E. Wales
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  2. Michael J. Eggertson
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  3. John R. Engen
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Editors and Affiliations

  1. Inst. Molecular Pathology & Immunology, Universidade do Porto Inst. Molecular Pathology & Immunology, Porto, Portugal

    Rune Matthiesen

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Wales, T.E., Eggertson, M.J., Engen, J.R. (2013). Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data. In: Matthiesen, R. (eds) Mass Spectrometry Data Analysis in Proteomics. Methods in Molecular Biology, vol 1007. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-392-3_11

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  • DOI: https://doi.org/10.1007/978-1-62703-392-3_11

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-391-6

  • Online ISBN: 978-1-62703-392-3

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