Abstract
Patients with pemphigus have circulating anti-desmoglein (Dsg) 1 and/or Dsg3 autoantibodies that induce blister formation on the skin and mucous membrane. We describe here two assays that measure the pathogenic strength of autoantibodies in blister formation: an in vitro dissociation assay using primary human epidermal keratinocytes to assess pathogenicity of anti-Dsg3 autoantibodies, and an alternative method whereby anti-Dsg3 and Dsg1 autoantibodies are injected into organ-cultured human skin specimen.
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Acknowledgement
We thank Marwah Adly Saleh for helpful discussion.
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Ishii, K., Amagai, M. (2013). In Vitro Pathogenicity Assay for Anti-desmoglein Autoantibodies in Pemphigus. In: Has, C., Sitaru, C. (eds) Molecular Dermatology. Methods in Molecular Biology, vol 961. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-227-8_13
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DOI: https://doi.org/10.1007/978-1-62703-227-8_13
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-226-1
Online ISBN: 978-1-62703-227-8
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