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Single-Cell Analysis pp 211–235Cite as

Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 853))

Abstract

The immune system is a network of cells in which the constitutive members interact through dense and sometimes overlapping connections. The extreme complexity of this network poses a significant challenge for monitoring pathological conditions (e.g., food allergies, autoimmunity, and other chronic inflammatory diseases) and for discovering robust signatures of immunological responses that correlate with or predict the efficacy of interventions. The diversity among immune cells found in clinical samples (variations in cellular functions, lineages, and clonotypic breadth) requires approaches for monitoring immune responses with single-cell resolution.

In this chapter, we present an engineering approach for integrated single-cell analysis that uses interchangeable modular operations to provide a comprehensive characterization of the phenotypic, functional, and genetic variations for individual cells. We focus on the use of microfabricated devices to isolate and interrogate single cells, and on the analytical components that enable subsequent detection, correlation, and interpretation of multidimensional sets of data. We discuss specific challenges and opportunities in the realization of this concept, and review two examples where it has been implemented. The presented approach should provide a basis for the design and implementation of nonconventional bioanalytical processes for studying specific responses of an immune system.

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Authors and Affiliations

  1. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

    Ayça Yalçın

  2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

    Yvonne J. Yamanaka

  3. Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA

    J. Christopher Love

Authors
  1. Ayça Yalçın
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  2. Yvonne J. Yamanaka
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  3. J. Christopher Love
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Corresponding author

Correspondence to J. Christopher Love .

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Editors and Affiliations

  1. POOLIA Sweden, Warfvinges väg 20, Stockholm, 104 25, Sweden

    Sara Lindström

  2. , Division of Nanobiotechnology, Royal Institute of Technology, Roslagstullsbacken 21, Stockholm, 106 91, Sweden

    Helene Andersson-Svahn

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© 2012 Springer Science+Business Media, LLC

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Yalçın, A., Yamanaka, Y.J., Love, J.C. (2012). Analytical Technologies for Integrated Single-Cell Analysis of Human Immune Responses. In: Lindström, S., Andersson-Svahn, H. (eds) Single-Cell Analysis. Methods in Molecular Biology, vol 853. Humana Press. https://doi.org/10.1007/978-1-61779-567-1_16

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  • DOI: https://doi.org/10.1007/978-1-61779-567-1_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-566-4

  • Online ISBN: 978-1-61779-567-1

  • eBook Packages: Springer Protocols

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