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Detection and Quantitative Analysis of Small RNAs by PCR

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RNA Therapeutics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 629))

Abstract

Increasing lines of evidence indicate that small non-coding RNAs including miRNAs, piRNAs, rasiRNAs, 21U endo-siRNAs, and snoRNAs are involved in many critical biological processes. Functional studies of these small RNAs require a simple, sensitive, and reliable method for detecting and quantifying levels of small RNAs. Here, we describe such a method that has been widely used for the validation of cloned small RNAs and also for quantitative analyses of small RNAs in both tissues and cells.

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Acknowledgments

The authors would like to thank Jonathan Cho for reading and editing the text. This work was supported by grants from the National Institute of Health (HD048855 and HD050281) to W.Y.

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Authors and Affiliations

  1. Department of Physiology and Cell Biology, Anderson Biomedical Science, University of Nevada School of Medicine, Reno, NV, USA

    Seungil Ro & Wei Yan

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  1. Seungil Ro
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  2. Wei Yan
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Editors and Affiliations

  1. Institute for Cancer Research, Dept. Immunology, Norwegian Radium Hospital, Montebello, Oslo, 0310, Norway

    Mouldy Sioud

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Ro, S., Yan, W. (2010). Detection and Quantitative Analysis of Small RNAs by PCR. In: Sioud, M. (eds) RNA Therapeutics. Methods in Molecular Biology, vol 629. Humana Press. https://doi.org/10.1007/978-1-60761-657-3_19

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  • DOI: https://doi.org/10.1007/978-1-60761-657-3_19

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-656-6

  • Online ISBN: 978-1-60761-657-3

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