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Biological Microarrays pp 107–116Cite as

Lithographically Defined Two- and Three-Dimensional Tissue Microarrays

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 671))

Abstract

Traditional methods to study normal and pathological development of tissues have been limited by ­difficulties in controlling experimental conditions and quantifying biological processes of interest. Here we describe methods to create microarrays of engineered tissues that enable controlled and quantitative investigations. Using soft lithography-based techniques, extracellular matrix proteins can be microcontact printed or micromolded to make two- and three-dimensional micropatterned scaffolds. The ultimate form and resulting properties of the tissue construct are dictated by the geometry of the patterned extracellular matrix components. This chapter describes elastomeric stamp fabrication, microcontact printing and micromolding of extracellular matrix proteins, cell culture in micropatterned substrata, and quantitative immunofluorescence analysis of micropatterned tissues.

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Acknowledgments

This work was supported by grants from the NIH (CA128660 and GM083997), Susan G. Komen for the Cure (FAS0703855); and the David & Lucile Packard Foundation. C.M.N. holds a Career Award at the Scientific Interface from the Burroughs Wellcome Fund. E.W.G. was supported by post doctoral fellowships from the New Jersey Commission on Cancer Research and Susan G. Komen for the cure.

Author information

Authors and Affiliations

  1. Departments of Chemical Engineering and Molecular Biology, Princeton University, Princeton, NJ, USA

    Esther W. Gomez

  2. Department of Chemical Engineering, Princeton University, Princeton, NJ, USA

    Celeste M. Nelson

Authors
  1. Esther W. Gomez
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  2. Celeste M. Nelson
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Editor information

Editors and Affiliations

  1. , MIT Division of Health Sciences and Tech, Harvard Medical School, Landsdowne St. 65, Cambridge, 02139, Massachusetts, USA

    Ali Khademhosseini

  2. , Department of Mechanical and Aerospace E, Seoul National University, Kwanak-gu, Shinlim-dong 56-1-1, Seoul, 151-742, Korea, Republic of (South Korea)

    Kahp-Yang Suh

  3. Biophage Pharma, Inc., Royalmount Ave. 6100, Montreal, H4P 2R2, Québec, Canada

    Mohammed Zourob

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Gomez, E.W., Nelson, C.M. (2011). Lithographically Defined Two- and Three-Dimensional Tissue Microarrays. In: Khademhosseini, A., Suh, KY., Zourob, M. (eds) Biological Microarrays. Methods in Molecular Biology, vol 671. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-551-0_5

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  • DOI: https://doi.org/10.1007/978-1-59745-551-0_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-95-4

  • Online ISBN: 978-1-59745-551-0

  • eBook Packages: Springer Protocols

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