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Overexpression of Chimeric RNA by Retroviral Transduction

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Chimeric RNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2079))

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Abstract

Cloning the open reading frame (ORF) of a protein-coding chimeric RNA into a mammalian expression vector is a simple, practiced way to study the function of the chimeric RNA. Here, we provide procedures for the insertion of the RRM2-C2orf48 fusion ORF into a mammalian expression vector, achieving the overexpression of the RRM2-C2orf48 fusion protein in a colon cancer cell line by retroviral transduction.

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Author information

Authors and Affiliations

  1. Department of Gastrointestinal Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China

    Hao Wu

  2. Department of Pathology, School of Medicine, University of Virginia, Charlottesville, VA, USA

    Hao Wu & Hui Li

  3. Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA, USA

    Hui Li

Authors
  1. Hao Wu
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  2. Hui Li
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Corresponding author

Correspondence to Hui Li .

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Editors and Affiliations

  1. Department of Paathology, School of Medicine, University of Virginia, Charlottesville, VA, USA, Department of Biochemistry and Molecular Genetics, School of Medicine, University of Virginia, Charlottesville, VA, USA

    Hui Li

  2. Department of Biochemistry and Molecular Genetics, University of Virginia, Charlottesville, VA, USA

    Justin Elfman

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Wu, H., Li, H. (2020). Overexpression of Chimeric RNA by Retroviral Transduction. In: Li, H., Elfman, J. (eds) Chimeric RNA. Methods in Molecular Biology, vol 2079. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9904-0_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9904-0_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9903-3

  • Online ISBN: 978-1-4939-9904-0

  • eBook Packages: Springer Protocols

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