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Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies

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Genotype Phenotype Coupling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2070))

Abstract

Monoclonal antibodies are among the most significant biological tools used in medicine and biology that have revolutionized the field of diagnostics, therapeutics, and targeted drug delivery systems for many diseases. Among them, rabbit monoclonal antibodies have attracted significant attention for having high affinity and specificity. During the past few decades, different techniques have been developed to produce monoclonal antibodies. Single B cell cloning technology offers many advantages compared to other methods and has been used to generate monoclonal antibodies from different species including rabbits. This review briefly describes some of these methods, with main focus on single B cell cloning and production of rabbit monoclonal antibodies.

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Authors and Affiliations

  1. Cell Marque/MilliporeSigma, Rocklin, CA, USA

    Juliet Rashidian & Joshua Lloyd

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  1. Juliet Rashidian
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  2. Joshua Lloyd
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Correspondence to Juliet Rashidian .

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Editors and Affiliations

  1. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Stefan Zielonka

  2. Protein Engineering and Antibody Technologies, Merck KGaA, Darmstadt, Germany

    Simon Krah

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Rashidian, J., Lloyd, J. (2020). Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies. In: Zielonka, S., Krah, S. (eds) Genotype Phenotype Coupling. Methods in Molecular Biology, vol 2070. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9853-1_23

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  • DOI: https://doi.org/10.1007/978-1-4939-9853-1_23

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9852-4

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