Abstract
Individual age is a phenotypic trait that provides useful information not only in forensic investigations but also in the aging research which is becoming an urgent call due to the dramatic growth of the aging population worldwide.
TaqMan quantification PCR (qPCR) can be successfully applied to biological age estimation, using method defined in Zubakov et al. (Curr Biol 20:R970-R971, 2010). Since levels of signal joint T-cell receptor rearrangement excision circle (sjTREC) in human lymphocytes are known to decrease with age increasing, the qPCR of sjTREC represents a simple and relatively reproducible technique which offers highly accurate age estimation results.
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References
Douek DC, McFarland RD, Keiser PH et al (1998) Changes in thymic function with age and during the treatment of HIV infection. Nature 396:690–695. https://doi.org/10.1038/25374
Zubakov D, Liu F, van Zelm MC et al (2010) Estimating human age from T-cell DNA rearrangements. Curr Biol 20:R970–R971. https://doi.org/10.1016/j.cub.2010.10.022
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Castagnetta, M. et al. (2020). qPCR Applications for the Determination of the Biological Age. In: Biassoni, R., Raso, A. (eds) Quantitative Real-Time PCR. Methods in Molecular Biology, vol 2065. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9833-3_14
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DOI: https://doi.org/10.1007/978-1-4939-9833-3_14
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Publisher Name: Humana, New York, NY
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