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Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Tissue engineering technologies have produced controllable and reproducible three-dimensional (3D) models that mimic the architecture and complexity of native tissues. In particular cell biology-based research is driven by the development of cell culture platforms and techniques that allow the analysis of cells cultured in 3D. Here we summarize several easy-to-follow methods for the characterization of cells that have been encapsulated and grown in hydrogels to measure their cell viability, metabolic activity, and mechanical properties of cell-containing hydrogels. We also describe an enzymatic approach for the digestion of cell-containing hydrogels and cell recovery thereby maintaining high cell viability for subsequent analysis.

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Acknowledgements

We thank Dr. Michelle Lockley for providing Ovcar-4 cells, Dr. Laura Lecker for her assistance and guidance with the GelMa synthesis, and acknowledge the technical assistance of the microscopy and flow cytometry facilities.

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Authors and Affiliations

  1. Centre for Cancer and Inflammation, Barts Cancer Institute, Queen Mary University of London, London, UK

    Eleonora Peerani, Juliana B. Candido & Daniela Loessner

  2. Queensland University of Technology, Brisbane, QLD, Australia

    Daniela Loessner

Authors
  1. Eleonora Peerani
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  2. Juliana B. Candido
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  3. Daniela Loessner
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Corresponding author

Correspondence to Daniela Loessner .

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Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Peerani, E., Candido, J.B., Loessner, D. (2019). Cell Recovery of Hydrogel-Encapsulated Cells for Molecular Analysis. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

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