Abstract
Xenopus oocytes serve as a standard heterologous expression system for the study of various ligand-gated ion channels including ATP P2X receptors. Here we describe the whole-cell two-electrode voltage clamp and biotinylation/Western blotting techniques to investigate the functional properties and surface trafficking from P2X-expressing oocytes.
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Bertin, E., Martínez, A., Boué-Grabot, E. (2020). P2X Electrophysiology and Surface Trafficking in Xenopus Oocytes. In: Pelegrín, P. (eds) Purinergic Signaling. Methods in Molecular Biology, vol 2041. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9717-6_18
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DOI: https://doi.org/10.1007/978-1-4939-9717-6_18
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