Abstract
ADAMTS7 is a secreted protease that is predominantly expressed in tissues of the cardiovascular system and tendon. Although recent evidence suggests that it may play a role in the etiology of coronary artery disease, its physiological function and substrates are unknown. The enzyme undergoes extensive posttranslational modifications, including chondroitin sulfate attachment, N and O-linked glycosylation, and a two-step activation process. For the benefit of scientists who study the function of ADAMTS7 and its role in disease, this chapter provides an introduction to the chemical and functional properties of the various ADAMTS7 domains, as well as a protocol for the recombinant expression and purification of ADAMTS7.
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Acknowledgments
This work was supported by a British Heart Foundation grant awarded to Rens de Groot (PG/18/19/33584). Dirk Wildeboer, Danielle Egan, and Salvatore Santamaria provided valuable feedback on the manuscript.
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de Groot, R. (2020). ADAMTS7: Recombinant Protein Expression and Purification. In: Apte, S. (eds) ADAMTS Proteases. Methods in Molecular Biology, vol 2043. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9698-8_6
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DOI: https://doi.org/10.1007/978-1-4939-9698-8_6
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