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G-Quadruplex Nucleic Acids pp 383–395Cite as

G-Quadruplex Visualization in Cells via Antibody and Fluorescence Probe

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2035))

Abstract

G-quadruplexes (G4s) are noncanonical nucleic acids structures involved in key regulatory and pathological roles in eukaryotes, prokaryotes, and viruses: the development of specific antibodies and fluorescent probes represent an invaluable tool to understand their biological relevance. We here present three protocols for the visualization of G4s in cells, both uninfected and HSV-1 infected, using a specific antibody and a fluorescent G4 ligand, and the effect of the fluorescent ligand on a G4 binding protein, nucleolin, upon binding of the molecule to the nucleic acids structure.

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Authors and Affiliations

  1. Department of Molecular Medicine, University of Padua, Padua, Italy

    Matteo Nadai & Sara N. Richter

Authors
  1. Matteo Nadai
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  2. Sara N. Richter
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Corresponding author

Correspondence to Sara N. Richter .

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Editors and Affiliations

  1. Department of Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, Purdue Center for Cancer Research, Purdue Institute for Drug Discovery, West Lafayette, IN, USA

    Danzhou Yang

  2. Medicinal Chemistry and Molecular Pharmacology, College of Pharmacy, Purdue University, West Lafayette, IN, USA

    Clement Lin

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Nadai, M., Richter, S.N. (2019). G-Quadruplex Visualization in Cells via Antibody and Fluorescence Probe. In: Yang, D., Lin, C. (eds) G-Quadruplex Nucleic Acids. Methods in Molecular Biology, vol 2035. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9666-7_24

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  • DOI: https://doi.org/10.1007/978-1-4939-9666-7_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9665-0

  • Online ISBN: 978-1-4939-9666-7

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