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High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research

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High-Throughput Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1978))

Abstract

Metabolomics based on direct mass spectrometry analysis shows a great potential in biomedical research because of its high-throughput screening capability and wide metabolome coverage. This chapter contains detailed protocols to perform comprehensive metabolomic fingerprinting of multiple biological samples (serum, plasma, urine, brain, liver, spleen, thymus) by using complementary analytical platforms. The most important issues to be considered are discussed, including sample treatment, metabolomic analysis, raw data preprocessing, and data analysis.

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Author information

Authors and Affiliations

  1. Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Huelva, Spain

    Raúl González-Domínguez, Ana Sayago & Ángeles Fernández-Recamales

  2. International Campus of Excellence CeiA3, University of Huelva, Huelva, Spain

    Raúl González-Domínguez, Ana Sayago & Ángeles Fernández-Recamales

  3. Department of Pediatrics, Hospital Universitario Puerta del Mar, Cádiz, Spain

    Álvaro González-Domínguez, Rosa María Mateos & Alfonso María Lechuga-Sancho

  4. Institute of Research and Innovation in Biomedical Sciences of the Province of Cádiz (INiBICA), Cádiz, Spain

    Álvaro González-Domínguez, Carmen Segundo, Mónica Schwarz, Rosa María Mateos & Alfonso María Lechuga-Sancho

  5. “Salus Infirmorum” Faculty of Nursing, University of Cádiz, Cádiz, Spain

    Carmen Segundo & Mónica Schwarz

  6. Instituto de Investigación Vitivinícola y Agroalimentario (IVAGRO), University of Cádiz, Puerto Real, Spain

    Mónica Schwarz & Enrique Durán-Guerrero

  7. Department of Analytical Chemistry, University of Cádiz, Puerto Real, Spain

    Enrique Durán-Guerrero

  8. Department of Mother and Child Health and Radiology, Faculty of Medicine, University of Cádiz, Cádiz, Spain

    Alfonso María Lechuga-Sancho

Authors
  1. Raúl González-Domínguez
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  2. Álvaro González-Domínguez
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  3. Carmen Segundo
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  4. Mónica Schwarz
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  5. Ana Sayago
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  6. Rosa María Mateos
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  7. Enrique Durán-Guerrero
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  8. Alfonso María Lechuga-Sancho
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  9. Ángeles Fernández-Recamales
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Corresponding author

Correspondence to Raúl González-Domínguez .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, University of Colorado Denver, Aurora, CO, USA

    Angelo D'Alessandro

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González-Domínguez, R. et al. (2019). High-Throughput Metabolomics Based on Direct Mass Spectrometry Analysis in Biomedical Research. In: D'Alessandro, A. (eds) High-Throughput Metabolomics. Methods in Molecular Biology, vol 1978. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9236-2_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9236-2_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9235-5

  • Online ISBN: 978-1-4939-9236-2

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