Abstract
Macrophages are cells of the immune system that have been suggested as important regulators of whole-body metabolism in mammals. In obesity, adipose tissue macrophages (ATMs) are thought to play both a detrimental and a beneficial role in the regulation of insulin sensitivity. Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in ATMs in obese mice. Using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by ATMs in the regulation of metabolism.
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Acknowledgment
We would like to thank Tarja Schröder for the tissue histology and Kjell Hultenby for the electron microscopy. This work was supported by research grants from AstraZeneca through the ICMC (M.A.), the Swedish Research Council (M.A.: 2015-03582), and the Novo Nordisk Foundation, including the Tripartite Immuno-metabolism Consortium (M.A. and TrIC; NNF15CC0018486) and the Strategic Research Program in Diabetes at Karolinska Institutet (M.A.).
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Barreby, E., Sulen, A., Aouadi, M. (2019). Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages. In: Gage, M., Pineda-Torra, I. (eds) Lipid-Activated Nuclear Receptors. Methods in Molecular Biology, vol 1951. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9130-3_4
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DOI: https://doi.org/10.1007/978-1-4939-9130-3_4
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-9130-3
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