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Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection

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Lipid-Activated Nuclear Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1951))

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Abstract

Macrophages are phagocytic cells that actively engulf and kill microorganisms within a specialized phagolysosomal system. Several pathogenic bacteria, however, actively co-opt host mechanisms and escape from microbial digestion to establish intracellular replication within macrophages. This chapter highlights detailed protocols to measure the effects of the LXR pathway on bacterial infection of murine bone marrow-derived macrophages.

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References

  1. Flannagan RS, Cosío G, Grinstein S (2009) Antimicrobial mechanisms of phagocytes and bacterial evasion strategies. Nat Rev Microbiol 7:355–366

    Article  CAS  Google Scholar 

  2. Price JV, Vance RE (2014) The Macrophage Paradox. Immunity 41:685–693

    Article  CAS  Google Scholar 

  3. Guiney DG, Lesnick M (2005) Targeting of the actin cytoskeleton during infection by Salmonella strains. Clin Immunol 114:248–255

    Article  CAS  Google Scholar 

  4. Haraga A, Ohlson MB, Miller SI (2008) Salmonellae interplay with host cells. Nat Rev Microbiol 6:53–66

    Article  CAS  Google Scholar 

  5. Hong C, Tontonoz P (2014) Liver X receptors in lipid metabolism: opportunities for drug discovery. Nat Rev Drug Discov 13:433–444

    Article  CAS  Google Scholar 

  6. Pascual-García M, Valledor AF (2012) Biological roles of liver x receptors in immune cells. Arch Immunol Ther Exp 60:235–249

    Article  Google Scholar 

  7. Matalonga J, Glaria E, Bresque M et al (2017) The Nuclear Receptor LXR Limits Bacterial Infection of Host Macrophages through a Mechanism that Impacts Cellular NAD Metabolism. Cell Rep 18:1241–1255

    Article  CAS  Google Scholar 

  8. Valledor AF, Comalada M, Xaus J et al (2000) The differential time-course of extracellular-regulated kinase activity correlates with the macrophage response toward proliferation or activation. J Biol Chem 275:7403–7409

    Article  CAS  Google Scholar 

  9. Birmingham CL, Smith AC, Bakowski MA et al (2006) Autophagy controls Salmonella infection in response to damage to the Salmonella-containing vacuole. J Biol Chem 281:11374–11383

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant from the Spanish Ministry of Economy and Competitivity (SAF2017-89510-R) to A.F. Valledor. Estibaliz Glaría is supported by a fellowship from the University of Barcelona (Ajuts de Personal Investigador predoctoral en Formació, APIF).

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Authors and Affiliations

  1. Nuclear Receptor Group, Department of Cell Biology, Physiology and Immunology, School of Biology, University of Barcelona, Barcelona, Spain

    Estibaliz Glaría, Jonathan Matalonga & Annabel F. Valledor

  2. Institute of Biomedicine of the University of Barcelona (IBUB), Barcelona, Spain

    Estibaliz Glaría & Annabel F. Valledor

Authors
  1. Estibaliz Glaría
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  2. Jonathan Matalonga
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  3. Annabel F. Valledor
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Corresponding author

Correspondence to Annabel F. Valledor .

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Editors and Affiliations

  1. Division of Medicine, Centre for Cardiometabolic Medicine, University College London, London, UK

    Matthew C. Gage

  2. Division of Medicine, Centre for Cardiometabolic Medicine, University College London, London, UK

    Inés Pineda-Torra

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Glaría, E., Matalonga, J., Valledor, A.F. (2019). Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection. In: Gage, M., Pineda-Torra, I. (eds) Lipid-Activated Nuclear Receptors. Methods in Molecular Biology, vol 1951. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9130-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-9130-3_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9129-7

  • Online ISBN: 978-1-4939-9130-3

  • eBook Packages: Springer Protocols

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