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PREDECT Protocols for Complex 2D/3D Cultures

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Systems Chemical Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1888))

Abstract

PREDECT, a European IMI consortium, has assumed the task to generate robust 2D and 3D culture platforms. Protocols established for 2D and 3D monoculture and stromal coculture models of increasing complexity (spheroid, stirred-tank bioreactor, Matrigel- and collagen-embedded cultures) have been established between six laboratories within academia, biotech, and pharma. These models were tested using three tumor cell lines (MCF7, LNCaP, and NCI-H1437), covering three pathologies (breast, prostate, and lung), but should be readily transferable to other model systems. Fluorescent protein tagged cell lines were used for all platforms, allowing for online measurement of growth curves and drug responses to treatments. All methods, from culture setup to phenotypic characterization and gene expression profiling are described in this chapter.

The adaptable methodologies and detailed protocols described here should help to include these models more readily to the drug discovery pipeline.

Suzana Vidic, Marta F. Estrada, Kjersti Gjerde, Vítor E. Santo, Annika Osswald, and Michael Barbier share first authorship.

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Author information

Authors and Affiliations

  1. Faculty of Medicine, Institute of Biochemistry, University of Ljubljana, Ljubljana, Slovenia

    Suzana Vidic

  2. iBET, Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal

    Marta F. Estrada, Vítor E. Santo & Catarina Brito

  3. Janssen Pharmaceutica N.V, Beerse, Belgium

    Kjersti Gjerde & Ronald de Hoogt

  4. Boehringer Ingelheim RCV, GmbH & Co. KG, Vienna, Austria

    Annika Osswald

  5. Laboratory of Cell Biology and Histology, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium

    Michaël Barbier

  6. Recursion Pharmaceuticals, Salt Lake City, UT, USA

    Yolanda T. Chong

  7. Boehringer Ingelheim RCV, GmbH & Co. KG, Vienna, Austria

    Wolfgang Sommergruber

  8. Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal

    Marta F. Estrada, Vítor E. Santo & Catarina Brito

  9. Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach an der Riß, Germany

    Ralph Graeser

Authors
  1. Suzana Vidic
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  2. Marta F. Estrada
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  3. Kjersti Gjerde
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  4. Vítor E. Santo
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  5. Annika Osswald
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  6. Michaël Barbier
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  7. Yolanda T. Chong
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  8. Wolfgang Sommergruber
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  9. Ronald de Hoogt
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  10. Catarina Brito
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  11. Ralph Graeser
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Corresponding author

Correspondence to Ralph Graeser .

Editor information

Editors and Affiliations

  1. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Slava Ziegler

  2. Department of Chemical Biology, Max Planck Institute of Molecular Physiology, Dortmund, Germany

    Herbert Waldmann

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Vidic, S. et al. (2019). PREDECT Protocols for Complex 2D/3D Cultures. In: Ziegler, S., Waldmann, H. (eds) Systems Chemical Biology. Methods in Molecular Biology, vol 1888. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8891-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8891-4_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8890-7

  • Online ISBN: 978-1-4939-8891-4

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