Abstract
Numerous methods for the measurement of tacrolimus and cyclosporine A involving traditional liquid chromatography tandem mass spectrometry (LC-MS/MS) have previously been described. The majority of these methods use solid-phase extraction, liquid-liquid extraction, or protein precipitation extraction with instrument run times greater than 15 s per sample. Continued demands in clinical labs for greater efficiency and throughput have put increased stress on traditional technologies such as high-performance liquid chromatography-ultraviolet detection (HPLC-UV) and traditional LC-MS/MS. As an improvement to the existing methods, we describe a sensitive ultrafast LC-MS/MS with run times of less than 15 s per sample.
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Bjergum, M.W., Jannetto, P.J., Langman, L.J. (2019). Simultaneous Determination of Tacrolimus and Cyclosporine A in Whole Blood by Ultrafast LC-MS/MS. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 1872. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8823-5_11
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DOI: https://doi.org/10.1007/978-1-4939-8823-5_11
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-8823-5
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