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Functional Proteomics pp 83–92Cite as

A Protein Decomplexation Strategy in Snake Venom Proteomics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1871))

Abstract

Snake venoms are complex mixtures of proteins and peptides that play vital roles in the survival of venomous snakes. As with their diverse pharmacological activities, snake venoms can be highly variable, hence the importance of understanding the compositional details of different snake venoms. However, profiling venom protein mixtures is challenging, in particular when dealing with the diversity of protein subtypes and their abundances. Here we described an optimized strategy combining a protein decomplexation method with in-solution trypsin digestion and mass spectrometry of snake venom proteins. The approach involves the integrated use of C18 reverse-phase high-performance liquid chromatography (RP-HPLC), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and nano-electrospray ionization tandem mass spectrometry (nano-ESI-LC-MS/MS).

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Author information

Authors and Affiliations

  1. Venom Research and Toxicology Laboratory, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    Choo Hock Tan

  2. Protein and Interactomic Laboratory, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

    Kae Yi Tan & Nget Hong Tan

Authors
  1. Choo Hock Tan
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  2. Kae Yi Tan
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  3. Nget Hong Tan
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Corresponding author

Correspondence to Choo Hock Tan .

Editor information

Editors and Affiliations

  1. Array Bridge Inc., St. Louis, MO, USA

    Xing Wang

  2. Biotech Support Group LLC, Monmouth Junction, NJ, USA

    Matthew Kuruc

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Tan, C.H., Tan, K.Y., Tan, N.H. (2019). A Protein Decomplexation Strategy in Snake Venom Proteomics. In: Wang, X., Kuruc, M. (eds) Functional Proteomics. Methods in Molecular Biology, vol 1871. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8814-3_5

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  • DOI: https://doi.org/10.1007/978-1-4939-8814-3_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8813-6

  • Online ISBN: 978-1-4939-8814-3

  • eBook Packages: Springer Protocols

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