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Isolation and Characterization of Mouse Neutrophils

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Lung Innate Immunity and Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1809))

Abstract

Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.

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Author information

Authors and Affiliations

  1. Department of Medicine, University of Vermont College of Medicine, Burlington, VT, USA

    Niki D. J. Ubags & Benjamin T. Suratt

Authors
  1. Niki D. J. Ubags
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  2. Benjamin T. Suratt
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Corresponding author

Correspondence to Benjamin T. Suratt .

Editor information

Editors and Affiliations

  1. Department of Immunology and Microbiology University of Colorado School of Medicine, Department of Biomedical Research and Center for Genes Environment and Health, National Jewish Health Denver, CO, USA, Aurora,, Colorado, USA

    Scott Alper

  2. Division of Pulmonary Sciences and Critical Care Medicine Department of Medicine University of Colorado Denver, Division of Pulmonary, Critical Care and Sleep Medicine Department of Medicine National Jewish Health Denver, CO, USA, Aurora, Colorado, USA

    William J. Janssen

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Ubags, N.D.J., Suratt, B.T. (2018). Isolation and Characterization of Mouse Neutrophils. In: Alper, S., Janssen, W. (eds) Lung Innate Immunity and Inflammation. Methods in Molecular Biology, vol 1809. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8570-8_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8570-8_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8569-2

  • Online ISBN: 978-1-4939-8570-8

  • eBook Packages: Springer Protocols

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