Abstract
Tissue engineering aims to develop scaffolds that are biocompatible and mimic the mechanical and biological properties of the target tissue as closely as possible. Here, we describe the fabrication and characterization of a biodegradable, elastomeric porous scaffold: poly(1,8-octanediol-co-citric acid) (POC) incorporated with nanoscale hydroxyapatite (HA). While this chapter focuses on the scaffold’s potential for bone regeneration, POC can also be used in other tissue engineering applications requiring an elastomeric implant. Because of the relative ease with which POC can be synthesized, its mechanical properties can be tailored to mimic the structure and function of the target elastomeric tissue for enhanced tissue regeneration.
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References
O’Brien FJ (2011) Biomaterials and scaffolds for tissue engineering. Materials Today 14:88–95
Chung EJ, Sugimoto M, Ameer GA (2011) The role of hydroxyapatite in citric acid-based nanocomposites: surface characteristics, degradation, and osteogenicity in vitro. Acta Biomater 7:4057–4063
Yang J, Webb AR, Ameer GA (2004) Novel citric acid-based biodegradable elastomers for tissue engineering. Adv Mater 16:511–516
Chung EJ (2011) Poly(diol citrate)-hydroxyapatite nanocomposites for bone and ligament tissue engineering. Dissertation Northwestern University
Murugan R, Ramakrishna S (2005) Development of nanocomposites for bone grafting. Comp Sci Tech 65:2385–2406
Ignatius AA, Betz O, Augat P, Claes LE (2001) In vivo investigations on composites made of resorbable ceramics and poly(lactide) used as bone graft substitutes. J Biomed Mater Res 58:701–709
Rizzi SC, Heath DJ, Coombes AG, Bock N, Textor M, Downes S (2001) Biodegradable polymer/hydroxyapatite composites: surface analysis and initial attachment of human osteoblasts. J Biomed Mater Res 55(4):475–486
Qiu H, Yang J, Kodali P, Ameer GA (2006) A citric acid-based hydroxyapatite composite for orthopedic implants. Biomaterials 27:5845–5854
Móczó J, Pukánszky B (2008) Polymer micro and nanocomposites: structure, interactions, properties. J Indus Eng Chem 14:535–563
Sikavitsas VI, Temenoff JS, Mikos AG (2001) Biomaterials and bone mechanotransduction. Biomaterials 22:2581–2593
Temenoff JS, Lu L, Mikos AG (1999) Bone engineering. Em Squared, Toronto
Keaveny TM, Morgan EF, Niebur GL, Yeh OC (2001) Biomechanics of trabecular bone. Annu Rev Biomed Eng 3:307–333
Chung EJ, Sugimoto M, Koh JL, Ameer GA (2012) Low-pressure foaming: a novel method for the fabrication of porous scaffolds for tissue engineering. Tissue Eng Part C Methods 18:113–121
Chung EJ, Kodali P, Laskin W, Koh JL, Ameer GA (2011) Long-term in vivo response to citric acid-based nanocomposites for orthopaedic tissue engineering. J Mater Sci Mater Med 22:2131–2138
Chung EJ, Qiu H, Kodali P, Yang S, Sprague SM, Hwong J, Koh J, Ameer GA (2011) Early tissue response to citric acid-based micro- and nanocomposites. J Biomed Mater Res Part A 96:29–37
Acknowledgments
This work was supported by NIH grant R00HL124279 granted to EJC.
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Masehi-Lano, J.J., Chung, E.J. (2018). Engineering Citric Acid-Based Porous Scaffolds for Bone Regeneration. In: Chawla, K. (eds) Biomaterials for Tissue Engineering. Methods in Molecular Biology, vol 1758. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7741-3_1
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DOI: https://doi.org/10.1007/978-1-4939-7741-3_1
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