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Mouse Embryogenesis pp 177–190Cite as

Genome Editing During Development Using the CRISPR-Cas Technology

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1752))

Abstract

Over the years, the study of gene function during development involved the implementation of sophisticated transgenic strategies to visualize how organisms change during their lifetime. These strategies are diverse and extremely useful and allowed the discovery of some of the fundamental mechanisms governing organism’s development. Such strategies can be time-consuming, in some cases expensive, and require complex infrastructure. With the advent of the genome editing CRISPR-Cas9 RNA-guided DNA endonuclease system a tremendous progress has been achieved in manipulating diverse organisms and cell types. In recent years this system has contributed importantly to the design of novel experimental strategies to further understand developmental processes, to generate genetically modified animal models, and develop disease models. Here we highlight examples in which the genome editing CRISPR-Cas9 system has been employed to understand the mechanisms controlling embryonic development and disease.

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Acknowledgments

This work was supported by the DGAPA-PAPIIT, UNAM (IN209403, IN203811, and IN201114), CONACyT (42653-Q, 128464, and 220503) and Fronteras de la Ciencia-2015 (Grant 290) to FR-T. DGAPA-PAPIIT, UNAM (IA202116) and CONACyT (CB-2015-01-255287, S0008-2015-2-261227, and INFR-2017-01-280464) to PL-L. RGA-M is doctoral student from Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (UNAM) and is recipient of the fellowship 317534 and 25590 (Fronteras) from CONACyT.

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Authors and Affiliations

  1. Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México

    Rodrigo G. Arzate-Mejía & Félix Recillas-Targa

  2. Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad de México, México

    Paula Licona-Limón

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  1. Rodrigo G. Arzate-Mejía
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  2. Paula Licona-Limón
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  3. Félix Recillas-Targa
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Correspondence to Félix Recillas-Targa .

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  1. Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada

    Paul Delgado-Olguin

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Arzate-Mejía, R.G., Licona-Limón, P., Recillas-Targa, F. (2018). Genome Editing During Development Using the CRISPR-Cas Technology. In: Delgado-Olguin, P. (eds) Mouse Embryogenesis. Methods in Molecular Biology, vol 1752. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7714-7_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7714-7_16

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