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Metabolic Profiling pp 225–235Cite as

LC-MS Untargeted Protocol for the Analysis of Wine

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1738))

Abstract

This chapter describes a protocol for the analysis of the metabolomic fingerprint of wine by liquid chromatography-mass spectrometry. The straightforward, optimized sample preparation procedure is limited to a single-step dilution with water or acetonitrile. The separation of wine analytes is carried out by two columns with orthogonal selectivity, including both reversed-phase (C18) and hydrophilic interaction (HILIC) chromatography, while the detection is assured by a high-resolution quadrupole time-of-flight mass spectrometer operating in negative and positive electrospray ionization mode, in order to obtain four different chromatograms for each sample. This validated protocol, or parts of it, could be applied in several oenological topic experimental designs, including wine quality and wine authenticity.

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Author information

Authors and Affiliations

  1. Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele all’Adige, Italy

    Panagiotis Arapitsas & Fulvio Mattivi

  2. Center Agriculture Food Environment, University of Trento, San Michele all’Adige, Italy

    Fulvio Mattivi

Authors
  1. Panagiotis Arapitsas
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  2. Fulvio Mattivi
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Corresponding author

Correspondence to Panagiotis Arapitsas .

Editor information

Editors and Affiliations

  1. Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Georgios A. Theodoridis

  2. School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Helen G. Gika

  3. Department of Surgery and Cancer, Imperial College London, London, United Kingdom

    Ian D. Wilson

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Arapitsas, P., Mattivi, F. (2018). LC-MS Untargeted Protocol for the Analysis of Wine. In: Theodoridis, G., Gika, H., Wilson, I. (eds) Metabolic Profiling. Methods in Molecular Biology, vol 1738. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7643-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-7643-0_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7642-3

  • Online ISBN: 978-1-4939-7643-0

  • eBook Packages: Springer Protocols

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