Abstract
MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene targeted enrichment sequencing, and computational analysis based on comparative genomics are provided in this chapter.
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Acknowledgements
The authors thank the support by the Gatsby Charitable Foundation, UK, the Two Blades Foundation, USA and the Biotechnology and Biological Sciences Research Council, UK, Grant numbers BB/H019820/1, BB/L009293/1 and BB/L011794/1.
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Steuernagel, B., Witek, K., Jones, J.D.G., Wulff, B.B.H. (2017). MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes. In: Periyannan, S. (eds) Wheat Rust Diseases. Methods in Molecular Biology, vol 1659. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7249-4_19
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DOI: https://doi.org/10.1007/978-1-4939-7249-4_19
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