Abstract
With the advent of “–omics” technologies there has been an explosion of data generation in the field of toxicology, as well as many others. As new candidate biomarkers of toxicity are being regularly discovered, the next challenge is to validate these observations in a targeted manner. Traditionally, these validation experiments have been conducted using antibody-based technologies such as Western blotting, ELISA, and immunohistochemistry. However, this often produces a significant bottleneck as the time, cost, and development of successful antibodies are often far outpaced by the generation of targets of interest. In response to this, there recently have been several developments in the use of triple quadrupole (QQQ) mass spectrometry (MS) as a platform to provide quantification of proteins. This technology does not require antibodies; it is typically less expensive and quicker to develop assays and has the opportunity for more accessible multiplexing. The speed of these experiments combined with their flexibility and ability to multiplex assays makes the technique a valuable strategy to validate biomarker discovery.
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Acknowledgments
The authors would like to thank Agilent Technologies, Santa Clara, for generating much of the data and figures used in this example. We would like to also thank all members of the PredTox Consortium. Funding is acknowledged under the FP6 Integrated Project, InnoMed. The UCD Conway Institute and the Proteome Research Centre is funded by the Programme for Research in Third Level Institutions (PRTLI), as administered by the Higher Education Authority (HEA) of Ireland.
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Lau, T.Y.K., Collins, B.C., Stone, P., Tang, N., Gallagher, W.M., Pennington, S.R. (2017). Absolute Quantification of Toxicological Biomarkers via Mass Spectrometry. In: Gautier, JC. (eds) Drug Safety Evaluation. Methods in Molecular Biology, vol 1641. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7172-5_19
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DOI: https://doi.org/10.1007/978-1-4939-7172-5_19
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