Abstract
Fourier transform infrared spectroscopy (FT-IR) was used to scan whole bacterial cells as well as lipopolysaccharides (LPSs, endotoxins) isolated from them. Proteus mirabilis cells, with chemically defined LPSs, served as a model for the ATR FT-IR method. The paper focuses on three steps of infrared spectroscopy: (1) sample preparation, (2) IR scanning, and (3) multivariate analysis of IR data (principal component analysis, PCA).
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References
Brunn GJ, Platt JL (2006) The etiology of sepsis: turned inside out. Trends Mol Med 12:10–16
Naumann D (2000) Infrared spectroscopy in microbiology. In: Meyers RA (ed) Encyclopedia of analytical chemistry: applications, theory, and instrumentation. John Wiley & Sons Ltd., Chichester, pp 102–131
Żarnowiec P, Lechowicz Ł, Czerwonka G, Kaca W (2015) Fourier Transform Infrared Spectroscopy (FTIR) as a tool for the identification and differentiation of pathogenic bacteria. Curr Med Chem 14:1710–1718
Davis R, Mauer L (2010) Fourier transform infrared (FT-IR) spectroscopy: a rapid tool for detection and analysis of foodborne pathogenic bacteria. Curr Res Technol Educ Top Appl Microbiol Microb Biotechnol II:1582–1594
Brandenburg K, Seydel U (1988) Orientation measurements on membrane systems made from lipopolysaccharides and free lipid A by FT-IR spectroscopy. Eur Biophys J 16:83–94
Seydel U, Koch C, Brandenburg K (1997) Investigations into lysozyme-endotoxin interactions with FT-IR spectroscopy and X-ray diffraction. Spectrosc Biol Mol Modem Trends:363–364
Zarnowiec P, Mizera A, Chrapek M et al (2016) Chemometric analysis of attenuated total reflectance infrared spectra of proteus mirabilis strains with defined structures of LPS. Innate Immun 22:325–335
Westphal O, Jann K, Himmelspach K (1983) Chemistry and immunochemistry of bacterial lipopolysaccharides as cell wall antigens and endotoxins. Prog Allergy 33:9–39
Galanos C, Luderitc O (1993) Isolation and precipitation of R-form of lipopolysaccharides: further applications of the PCP method. In: BeMiller J, Whistler R, Shaw D (eds) Methods carbohydr. Chem. John Wiley & Sons, Inc., pp 11–18
Gmeiner J (1975) The isolation of two different lipopolysaccharide fractions from various Proteus mirabilis strains. Eur J Biochem 626:621–626
Sousa C, Silva L, Grosso F et al (2014) Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218. J Photochem Photobiol B Biol 133:108–114
Winder CL, Goodacre R (2004) Comparison of diffuse-reflectance absorbance and attenuated total reflectance FT-IR for the discrimination of bacteria. Analyst 129:1118–1122
Nagib S, Rau J, Sammra O et al (2014) Identification of Trueperella pyogenes isolated from bovine mastitis by fourier transform infrared spectroscopy. PLoS One:9–e104654
Muhamadali H, Weaver D, Subaihi A et al (2015) Chicken, beams, and Campylobacter: rapid differentiation of foodborne bacteria via vibrational spectroscopy and MALDI-mass spectrometry. Analyst 141:111–122
Pupo E (2011) Isolation of smooth-type lipopolysaccharides to electrophoretic homogeneity. In: Holst O (ed) Microbial toxins: methods and protocols, Methods in molecular biology, vol 739. Springer New York, Totowa, NJ, pp 101–112
Brandes Ammann A, Brandl H (2011) Detection and differentiation of bacterial spores in a mineral matrix by Fourier transform infrared spectroscopy (FTIR) and chemometrical data treatment. BMC Biophys 4:14
Baker MJ, Trevisan J, Bassan P et al (2014) Using Fourier transform IR spectroscopy to analyze biological materials. Nat Protoc 9:1771–1791
Bosch A, Miñán A, Vescina C et al (2008) Fourier transform infrared spectroscopy for rapid identification of nonfermenting gram-negative bacteria isolated from sputum samples from cystic fibrosis patients. J Clin Microbiol 46:2535–2546
Baldauf NA, Rodriguez-Romo LA, Männig A et al (2007) Effect of selective growth media on the differentiation of Salmonella enterica serovars by Fourier-transform mid-Infrared spectroscopy. J Microbiol Methods 68:106–114
Al-Qadiri HM, Lin M, Cavinato AG, Rasco BA (2006) Fourier transform infrared spectroscopy, detection and identification of Escherichia coli O157:H7 and alicyclobacillus strains in apple juice. Int J Food Microbiol 111:73–80
Kim S, Reuhs BL, Mauer LJ (2005) Use of Fourier transform infrared spectra of crude bacterial lipopolysaccharides and chemometrics for differentiation of Salmonella enterica serotypes. J Appl Microbiol 99:411–417
Acknowledgments
This study was supported by grant from the National Science Center, Poland granted on the basis of decision number DEC-2012/07/N/NZ6/04118 and grant no. 612 427 from the Jan Kochanowski University. Some of the experiments were run on apparatus purchased with European Union grant 307 under 2.2 Innovation Industry.
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Żarnowiec, P., Czerwonka, G., Kaca, W. (2017). Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins. In: Holst, O. (eds) Microbial Toxins. Methods in Molecular Biology, vol 1600. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6958-6_11
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DOI: https://doi.org/10.1007/978-1-4939-6958-6_11
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