Abstract
Most of the research on the histamine receptors in in vitro studies was accomplished using recombinant systems. These biological models proved to be ideal in the determination of the receptors’ function, biochemistry, and molecular signaling pathways. Unfortunately, they do not reflect the real biological situation, as the receptors are artificially introduced to host system and usually expressed in high quantity, not regulated as in native cells. Recently in order to overcome the limitations of recombinant systems, several important works were published that focus on the histamine receptors’ function in native human cells. For histamine H4 receptor functional studies immune cells, including human eosinophils, were mainly used. Therefore in this chapter we discuss the tools and provide step-by-step protocols used in the pharmacological evaluation of histamine H4 receptor function in primary human eosinophils. Methods include: isolation of human eosinophils from peripheral blood, functional determination of the effects of histamine receptors’ ligands (including selective histamine receptor agonists and antagonists), and data elaboration. We believe that the described methods may play an important role in future studies because as yet the function of histamine receptors on the other immune cells remains elusive.
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Acknowledgments
This work was supported by the Polish National Science Center grant: “Research on human eosinophils as a potential therapeutic target” DEC/2014/13/N/NZ7/00897 and “Histamine H3/H4 receptors as an attractive target for the search of biologically active compounds” DEC/2011/02/A/NZ4/00031.
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Grosicki, M., Kieć-Kononowicz, K. (2017). Techniques Used in Pharmacological Evaluation of Histamine H4 Receptor Function on Native Human Eosinophils. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_8
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DOI: https://doi.org/10.1007/978-1-4939-6843-5_8
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