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Light Microscopy pp 1–15Cite as

Introduction to Modern Methods in Light Microscopy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

For centuries, light microscopy has been a key method in biological research, from the early work of Robert Hooke describing biological organisms as cells, to the latest in live-cell and single-molecule systems. Here, we introduce some of the key concepts related to the development and implementation of modern microscopy techniques. We briefly discuss the basics of optics in the microscope, super-resolution imaging, quantitative image analysis, live-cell imaging, and provide an outlook on active research areas pertaining to light microscopy.

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Author information

Authors and Affiliations

  1. LMU Munich, Biocenter Martinsried, Grosshadernerstr. 2, 82152, Martinsried, Munich, Germany

    Joel Ryan

  2. Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, QC, Canada

    Abby R. Gerhold

  3. University of North Carolina at Chapel Hill, Chapel Hill, NC, USA

    Vincent Boudreau & Lydia Smith

  4. Department of Biology, University of North Carolina at Chapel Hill, 4358 Genome Sciences Building, Chapel Hill, NC, 27599, USA

    Paul S. Maddox

Authors
  1. Joel Ryan
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  2. Abby R. Gerhold
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  3. Vincent Boudreau
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  4. Lydia Smith
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  5. Paul S. Maddox
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Corresponding author

Correspondence to Paul S. Maddox .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

    Yolanda Markaki

  2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

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Ryan, J., Gerhold, A.R., Boudreau, V., Smith, L., Maddox, P.S. (2017). Introduction to Modern Methods in Light Microscopy. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6808-4

  • Online ISBN: 978-1-4939-6810-7

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