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Assessment of Caveolae/Lipid Rafts in Isolated Cells

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Hypertension

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1527))

Abstract

This chapter outlines protocols to evaluate protein localization, recruitment or phosphorylation levels in cholesterol/sphingolipids-enriched cell membrane domains and recommends experimental designs with pharmacological tolls to evaluate potential cell functions associated with these domains. We emphasize the need for the combination of several approaches towards understanding the protein components and cellular functions attributed to these distinct microdomains.

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Author information

Authors and Affiliations

  1. Kidney Research Centre, Department of Medicine, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada

    G. E. Callera, Thiago Bruder-Nascimento & R. M. Touyz

  2. Department of Pharmacology, Medical School of Ribeirao Preto, University of Sao Paulo, Sao Paulo, Brazil

    Thiago Bruder-Nascimento

  3. Institute of Cardiovascular & Medical Sciences, BHF Glasgow Cardiovascular Research Centre, University of Glasgow, 126 University Place, Glasgow, G12 8TA, Scotland, UK

    R. M. Touyz

Authors
  1. G. E. Callera
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  2. Thiago Bruder-Nascimento
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  3. R. M. Touyz
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Corresponding author

Correspondence to R. M. Touyz .

Editor information

Editors and Affiliations

  1. Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom

    Rhian M. Touyz

  2. Lady Davis Institute for Medical Research and Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada

    Ernesto L. Schiffrin

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Callera, G.E., Bruder-Nascimento, T., Touyz, R.M. (2017). Assessment of Caveolae/Lipid Rafts in Isolated Cells. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_20

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  • DOI: https://doi.org/10.1007/978-1-4939-6625-7_20

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6623-3

  • Online ISBN: 978-1-4939-6625-7

  • eBook Packages: Springer Protocols

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