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Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR

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Drug Target miRNA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1517))

Abstract

Identification and validation of microRNA (miRNA) target genes is essential for gaining a better understanding of the many different functions miRNAs have in healthy and diseased cells. From a practical standpoint, validated target genes are also useful for monitoring pharmacological activity of developmental therapeutics that modulate miRNAs, such as anti-miRNA oligonucleotides (anti-miR). Here, we describe a method that uses changes in Argonaute 2-RNA immunoprecipitation in response to competition by anti-miR, titrated ex vivo, as physical evidence for target validation.

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Acknowledgement

This work was supported by Regulus Therapeutics Inc., which employed the author.

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Authors and Affiliations

  1. Regulus Therapeutics, Inc., 3545 John Hopkins Ct., Suite 210, San Diego, CA, 92121-1121, USA

    John R. Androsavich

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  1. John R. Androsavich
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Corresponding author

Correspondence to John R. Androsavich .

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Editors and Affiliations

  1. BEROCEUTICA GmbH, Potsdam, Germany

    Marco F. Schmidt

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Androsavich, J.R. (2017). Competitive Argonaute-Based RNA Immunoprecipitation for Investigation of Transcriptomic Response to Anti-miR. In: Schmidt, M. (eds) Drug Target miRNA. Methods in Molecular Biology, vol 1517. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6563-2_6

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  • DOI: https://doi.org/10.1007/978-1-4939-6563-2_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6561-8

  • Online ISBN: 978-1-4939-6563-2

  • eBook Packages: Springer Protocols

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