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Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing LacZ Reporters

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CCN Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1489))

Abstract

Two developments have greatly facilitated the construction of CCN mutant mouse strains. The first is the availability of modified embryonic stem (ES) cells and mice developed through several large-scale government-sponsored research programs. The second is the advent of CRISPR/Cas9 technology. In this chapter, we describe the available mouse strains generated by gene targeting techniques and the CCN targeting vectors and genetically modified ES cells that are available for the generation of CCN mutant mice. Many of these mutant mouse lines and ES cells carry a β-galactosidase reporter that can be used to track CCN expression, facilitating phenotypic analysis and revealing new sites of CCN action. Therefore, we also describe a method for β-galactosidase staining.

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Author information

Authors and Affiliations

  1. Department of Orthopaedic Surgery, School of Dentistry, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Jie Jiang Ph.D.

  2. Department of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Zhengshan Hu

  3. Department of Orthopaedic Surgery, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Karen M. Lyons Ph.D.

  4. Department of Molecular, Cell, & Developmental Biology, University of California Los Angeles, Los Angeles, CA, 90095, USA

    Karen M. Lyons Ph.D.

Authors
  1. Jie Jiang Ph.D.
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  2. Zhengshan Hu
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  3. Karen M. Lyons Ph.D.
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Corresponding author

Correspondence to Karen M. Lyons Ph.D. .

Editor information

Editors and Affiliations

  1. Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School/Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Masaharu Takigawa

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Jiang, J., Hu, Z., Lyons, K.M. (2017). Design and Analysis of CCN Gene Activity Using CCN Knockout Mice Containing LacZ Reporters. In: Takigawa, M. (eds) CCN Proteins. Methods in Molecular Biology, vol 1489. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6430-7_28

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  • DOI: https://doi.org/10.1007/978-1-4939-6430-7_28

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6428-4

  • Online ISBN: 978-1-4939-6430-7

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