Skip to main content

DNA Vaccination in Chickens

  • Protocol
  • First Online:
Book cover Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1404))

Abstract

Robust and sustainable development of poultry industry requires prevention of deadly infectious diseases. Vigorous vaccination of the birds is a routine practice; however, the live and inactivated vaccines that are used have inherent disadvantages. New-generation vaccines such as DNA vaccines offer several advantages over conventional vaccines. DNA vaccines, which encode an antigen of interest or multiple antigens in the target host, are stable, easy to produce and administer, do not require cold chain maintenance, and are not affected by the maternal antibodies. In addition, DNA vaccines can also be administered in ovo, and thus, mass vaccination and early induction of immune response can effectively be achieved. In this chapter, we focus on the development of DNA vaccines against important infectious viral as well as parasitic diseases of poultry.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Bowersock TL (2002) Evolving importance of biologics and novel delivery systems in the face of microbial resistance. AAPS PharmSci 4:1–7

    Google Scholar 

  2. O’Hagan DT, MacKichan ML, Singh M (2001) Recent developments in adjuvants for vaccines against infectious diseases. Biomol Eng 18:69–85

    PubMed  Google Scholar 

  3. Min W et al (2001) Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGFbeta4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 20:267–274

    CAS  PubMed  Google Scholar 

  4. Li K et al (2013) Adjuvant effects of interleukin-18 in DNA vaccination against infectious bursal disease virus in chickens. Vaccine 31:1799–1805

    CAS  PubMed  Google Scholar 

  5. Donnelly JJ, Ulmer JB, Shiver JW, Liu MA (1997) DNA vaccines. Annu Rev Immunol 15:617–648

    CAS  PubMed  Google Scholar 

  6. Dhama K, Mahendran M, Gupta PK, Rai A (2008) DNA vaccines and their applications in veterinary practice: current perspectives. Vet Res Commun 32:341–356

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Rao SS et al (2009) A gene-based avian influenza vaccine in poultry. Poultry Sci 88:860–866

    CAS  Google Scholar 

  8. Beard CW, Mason PW (1998) Out on the farm with DNA vaccines. Nat Biotechnol 16:1325–1328

    CAS  PubMed  Google Scholar 

  9. Van Duin D, Medzhitov R, Shaw AC (2006) Triggering TLR signaling in vaccination. Trends Immunol 27:49–55

    PubMed  Google Scholar 

  10. Coban C et al (2011) Novel strategies to improve DNA vaccine immunogenicity. Curr Gene Ther 11:479–484

    CAS  PubMed  Google Scholar 

  11. Gupta SK et al (2013) Recombinant flagellin and its cross-talk with lipopolysaccharide—effect on pooled chicken peripheral blood mononuclear cells. Res Vet Sci 95:930–935

    CAS  PubMed  Google Scholar 

  12. Gupta SK, Bajwa P, Deb R, Chellappa MM, Dey S (2014) Flagellin a toll-like receptor 5 agonist as an adjuvant in chicken vaccines. Clin Vaccine Immunol 21:261–270

    PubMed  PubMed Central  Google Scholar 

  13. Gupta SK, Deb R, Dey S, Chellappa MM (2014) Toll-like receptor-based adjuvants: enhancing the immune response to vaccines against infectious diseases of chicken. Exp Rev Vaccine 13:909–925

    CAS  Google Scholar 

  14. Loke CF, Omar AR, Raha AR, Yusoff K (2005) Improved protection from velogenic Newcastle disease virus challenge following multiple immunizations with plasmid DNA encoding for F and HN genes. Vet Immunol Immunopathol 106:259–267

    CAS  PubMed  Google Scholar 

  15. Sawant PM et al (2011) Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-γ and IL-4 genes. Vet Immunol Immunopathol 144:36–44

    CAS  PubMed  Google Scholar 

  16. Zhao K et al (2013) Preparation and efficacy of Newcastle disease virus DNA vaccine encapsulated in PLGA nanoparticles. PLoS One 8(12):e82648

    PubMed  PubMed Central  Google Scholar 

  17. Chang HC, Lin TL, Wu CC (2001) DNA-mediated vaccination against infectious bursal disease in chickens. Vaccine 20:328–335

    CAS  PubMed  Google Scholar 

  18. Hulse DJ, Romero CH (2004) Partial protection against infectious bursal disease virus through DNA-mediated vaccination with the VP2 capsid protein and chicken IL-2 genes. Vaccine 22:1249–1259

    CAS  PubMed  Google Scholar 

  19. Mahmood MS, Siddique M, Hussain I, Khan A, Mansoor MK (2006) Protection capability of recombinant plasmid DNA vaccine containing VP2 gene of very virulent infectious bursal disease virus in chickens adjuvanted with CpG oligodeoxynucleotide. Vaccine 24:4838–4846

    CAS  PubMed  Google Scholar 

  20. Park JH, Sung HW, Yoon BI, Kwon HM (2009) Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-γ. J Vet Sci 10:131–139

    PubMed  PubMed Central  Google Scholar 

  21. Kumar S et al (2009) Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2. Vaccine 27:864–869

    CAS  PubMed  Google Scholar 

  22. Sun JH, Yan YX, Jiang J, Lu P (2005) DNA immunization against very virulent infectious bursal disease virus with VP2‐4‐3 gene and chicken IL‐6 gene. J Vet Med B 52:1–7

    CAS  Google Scholar 

  23. Maity HK, Dey S, Mohan CM, Khulape SA, Pathak DC, Vakharia VN (2015) Protective efficacy of a DNA vaccine construct encoding the VP2 gene of infectious bursal disease and a truncated HSP70 of Mycobacterium tuberculosis in chickens. Vaccine 33:1033–1039

    CAS  PubMed  Google Scholar 

  24. Kapczynski DR, Hilt DA, Shapiro D, Sellers HS, Jackwood MW (2003) Protection of chickens from infectious bronchitis by in ovo and intramuscular vaccination with a DNA vaccine expressing the S1 glycoprotein. Avian Dis 47:272–285

    PubMed  Google Scholar 

  25. Tang M, Wang H, Zhou S, Tian G (2008) Enhancement of the immunogenicity of an infectious bronchitis virus DNA vaccine by a bicistronic plasmid encoding nucleocapsid protein and interleukin-2. J Virol Methods 149:42–48

    CAS  PubMed  PubMed Central  Google Scholar 

  26. Tan B, Wang H, Shang L, Yang T (2009) Coadministration of chicken GM-CSF with a DNA vaccine expressing infectious bronchitis virus (IBV) S1 glycoprotein enhances the specific immune response and protects against IBV infection. Arch Virol 154:1117–1124

    CAS  PubMed  Google Scholar 

  27. Yang T et al (2009) Multivalent DNA vaccine enhanced protection efficacy against infectious bronchitis virus in chickens. J Vet Med Sci 71:1585–1590

    CAS  PubMed  Google Scholar 

  28. Fynan EF, Robinson HL, Webster RG (1993) Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA Cell Biol 12:785–789

    CAS  PubMed  Google Scholar 

  29. Qiu M et al (2006) Protection against avian influenza H9N2 virus challenge by immunization with hemagglutinin-or neuraminidase-expressing DNA in BALB/c mice. Biochim Biophy Res Commun 343:1124–1131

    CAS  Google Scholar 

  30. Jiang Y (2007) Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector. Antiviral Res 75:234–241

    CAS  PubMed  Google Scholar 

  31. Rao S et al (2008) Multivalent HA DNA vaccination protects against highly pathogenic H5N1 avian influenza infection in chickens and mice. PLoS One 3(6):e2432

    PubMed  PubMed Central  Google Scholar 

  32. Jalilian B et al (2010) Research development of avian influenza virus H5 DNA vaccine and MDP-1 gene of Mycobacterium bovis as genetic adjuvant. Genet Vaccine Ther 8:4

    Google Scholar 

  33. Cherbonnel M, Rousset J, Jestin V (2003) Strategies to improve protection against low-pathogenicity H7 avian influenza virus infection using DNA vaccines. Avian Dis 47:1181–1186

    CAS  PubMed  Google Scholar 

  34. Oveissi S, Omar AR, Yusoff K, Jahanshiri F, Hassan SS (2010) DNA vaccine encoding avian influenza virus H5 and Esat-6 of Mycobacterium tuberculosis improved antibody responses against AIV in chickens. Comp Immunol Microbiol Infect Dis 33:491–503

    PubMed  Google Scholar 

  35. Sawant PM et al (2015) Development of a DNA vaccine for chicken infectious anemia and its immunogenicity studies using high mobility group box 1 protein as a novel immunoadjuvant indicated induction of promising protective immune responses. Vaccine 33:333–340

    CAS  PubMed  Google Scholar 

  36. Senthil K et al (2004) Development of DNA vaccine against chicken anemia virus simultaneously using its VP1 and VP2 proteins. Paper presented in XII Conference of IPSA and National Symposium, Himachal Pradesh Krishi Vishwavidalya, Palampur, p 152

    Google Scholar 

  37. Song KD et al (2000) A DNA vaccine encoding a conserved Eimeria protein induces protective immunity against live Eimeria acervulina challenge. Vaccine 19:243–252

    CAS  PubMed  Google Scholar 

  38. Ma D et al (2011) Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL-15 offers protection against homologous challenge. Exp Parasitol 127:208–214

    CAS  PubMed  Google Scholar 

  39. Shah MAA, Song X, Xu L, Yan R, Li X (2011) Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-γ and their efficacy against poultry coccidiosis. Res Vet Sci 90:72–77

    CAS  PubMed  Google Scholar 

  40. Ding X et al (2005) In ovo vaccination with the Eimeria tenella EtMIC2 gene induces protective immunity against coccidiosis. Vaccine 23:3733–3740

    CAS  PubMed  Google Scholar 

  41. Du A, Wang S (2005) Efficacy of a DNA vaccine delivered in attenuated Salmonella typhimurium against Eimeria tenella infection in chickens. Int J Parasitol 35:777–785

    CAS  PubMed  Google Scholar 

  42. Xu Q et al (2008) Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Vet Parasitol 156:319–323

    CAS  PubMed  Google Scholar 

  43. Cho SH, Loewen PC, Marquardt RR (2004) A plasmid DNA encoding chicken interleukin-6 and Escherichia coli K88 fimbrial protein FaeG stimulates the production of anti-K88 fimbrial antibodies in chickens. Poultry Sci 83:1973–1978

    CAS  Google Scholar 

  44. Brandsma JL (2006) DNA vaccine design. In: Saltzman MW, Shen H, Brandsma JL (eds) DNA vaccines. Humana, Totowa, NJ, pp 3–10

    Google Scholar 

  45. Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292

    CAS  PubMed  Google Scholar 

  46. Nagata T, Uchijima M, Yoshida A, Kawashima M, Koide Y (1999) Codon optimization effect on translational efficiency of DNA vaccine in mammalian cells: analysis of plasmid DNA encoding a CTL epitope derived from microorganisms. Biochim Biophys Res Commun 261:445–451

    CAS  Google Scholar 

  47. Ferraro B et al (2011) Clinical applications of DNA vaccines: current progress. Clin Infect Dis 53:296–302

    CAS  PubMed  PubMed Central  Google Scholar 

  48. Chang DZ, Lomazow W, Somberg CJ, Stan R, Perales MA (2004) Granulocyte-macrophage colony stimulating factor: an adjuvant for cancer vaccines. Hematology 9:207–215

    CAS  PubMed  Google Scholar 

  49. Mohan CM, Dey S, Rai A, Kataria JM (2006) Recombinant haemagglutinin neuraminidase antigen-based single serum dilution ELISA for rapid serological profiling of Newcastle disease virus. J Virol Methods 138:117–122

    CAS  PubMed  Google Scholar 

  50. Dey S et al (2004) Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis. Vet Microbiol 103:99–106

    CAS  PubMed  Google Scholar 

  51. Ruiz-Hernandez R et al (2014) An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination. J Immunol Methods 416:40–48

    PubMed  Google Scholar 

  52. Ariaans MP (2008) ELISPOT and intracellular cytokine staining: novel assays for quantifying T cell responses in the chicken. Dev Comp Immunol 32:1398–1404

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shishir Kumar Gupta .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Gupta, S.K., Dey, S., Chellappa, M.M. (2016). DNA Vaccination in Chickens. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3389-1_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-3388-4

  • Online ISBN: 978-1-4939-3389-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics