Abstract
Surface plasmon resonance (SPR) technique is extremely interesting in immunology because it has the potential to directly visualize biomolecular interactions in real-time monitoring antibody affinity, one of the parameters affecting pathogenicity in autoimmune diseases. Herein we describe the affinity evaluation of anti-citrullinated peptide antibodies (ACPA) to a peptide-based biosensor by SPR. The method describes the purification of ACPA isolated from rheumatoid arthritis (RA) patients using affinity columns, the strategy employed for the immobilization of citrullinated peptides onto a sensor chip, and the evaluation of the specific binding of purified ACPA to immobilized peptides.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kausaite A, Ramanaviciene A, Mostovojus V et al (2007) Surface plasmon resonance and its application to biomedical research. Medicina (Kaunas) 43:355–365
Kaymakcalan Z, Sakorafas P, Bose S et al (2009) Comparisons of affinities, avidities, and complement activation of adalimumab, infliximab, and etanercept in binding to soluble and membrane tumor necrosis factor. Clin Immunol 131:308–316
Wittenberg NJ, Wootla B, Jordan LR et al (2014) Applications of SPR for the characterization of molecules important in the pathogenesis and treatment of neurodegenerative diseases. Expert Rev Neurother 14:449–463
Hearty S, Conroy PJ, Ayyar BV et al (2010) Surface plasmon resonance for vaccine design and efficacy studies: recent applications and future trends. Expert Rev Vaccines 9:645–664
Cimitan S, Lindgren MT, Bertucci C et al (2005) Early absorption and distribution analysis of antitumor and anti-AIDS drugs: lipid membrane and plasma protein interactions. J Med Chem 48:3536–3546
Rossi G, Real-Fernández F, Panza F et al (2014) Biosensor analysis of anti-citrullinated protein/peptide antibody affinity. Anal Biochem 465C:96–101
van Beers JJ, Willemze A, Jansen JJ et al (2013) ACPA fine-specificity profiles in early rheumatoid arthritis patients do not correlate with clinical features at baseline or with disease progression. Arthritis Res Ther 15:R140
Suwannalai P, van de Stadt LA, Radner H et al (2012) Avidity maturation of anti-citrullinated protein antibodies in rheumatoid arthritis. Arthritis Rheum 64:1323–1328
Ossipova E, Cerqueira CF, Reed E et al (2014) Affinity purified anti-citrullinated protein/peptide antibodies target antigens expressed in the rheumatoid joint. Arthritis Res Ther 16:R167
Grodzki AC, Berenstein E (2010) Antibody purification: affinity chromatography—protein A and protein G Sepharose. Methods Mol Biol 588:33–41
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Real-Fernández, F., Rossi, G., Panza, F., Pratesi, F., Migliorini, P., Rovero, P. (2015). Surface Plasmon Resonance Method to Evaluate Anti-citrullinated Protein/Peptide Antibody Affinity to Citrullinated Peptides. In: Houen, G. (eds) Peptide Antibodies. Methods in Molecular Biology, vol 1348. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2999-3_23
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2999-3_23
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2998-6
Online ISBN: 978-1-4939-2999-3
eBook Packages: Springer Protocols