Abstract
Microfluidic platforms have greatly evolved in the last few years. This technology is nowadays transversely spread all over the most abroad fields of research. Compartmentalized microfluidic devices, initially applied in the neuroscience field for the simplest biochemical tests, is presently used at the most complex assays such as cocultures for developmental and regeneration studies. Excitingly, these devices have emerged as a potential tool to study, not only the interaction within central nervous system cells but also the innervation of peripheral tissues. This latest issue have led us to select the compartmentalized microfluidic devices to conduct the studies of peripheral neuro-osteogenic interactions. In this chapter we describe and standardize the major proceedings to ensure the success of the coculture model for neurons and osteoblasts and further recommend the qualitative and quantitative analysis for two- or three-dimensional cocultures.
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Acknowledgments
This work was financed by FEDER funds through the Programa Operacional Factores de Competitividade—COMPETE and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia in the framework of the projects PEst-C/SAU/LA0002/2013 and PTDC/BIM-MED/1047/2012. EN is recipient of PhD fellowship SFRH/BD/81152/2011.
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Neto, E., Paramos-de-Carvalho, D., Lourenço, A.H., Aguiar, P., Lamghari, M. (2015). Compartmentalized Microfluidic Platforms as Tool of Choice to Study the Interaction Between Neurons and Osteoblasts. In: Biffi, E. (eds) Microfluidic and Compartmentalized Platforms for Neurobiological Research. Neuromethods, vol 103. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2510-0_10
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DOI: https://doi.org/10.1007/978-1-4939-2510-0_10
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