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The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry with Particular Focus on Metabolomics Research

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Metabonomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1277))

Abstract

Mass spectrometry (MS) and nuclear magnetic resonance (NMR) have evolved as the most common techniques in metabolomics studies, and each brings its own advantages and limitations. Unlike MS spectrometry, NMR spectroscopy is quantitative and does not require extra steps for sample preparation, such as separation or derivatization. Although the sensitivity of NMR spectroscopy has increased enormously and improvements continue to emerge steadily, this remains a weak point for NMR compared with MS. MS-based metabolomics provides an excellent approach that can offer a combined sensitivity and selectivity platform for metabolomics research. Moreover, different MS approaches such as different ionization techniques and mass analyzer technology can be used in order to increase the number of metabolites that can be detected. In this chapter, the advantages, limitations, strengths, and weaknesses of NMR and MS as tools applicable to metabolomics research are highlighted.

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Acknowledgments

We would like to thank King Abdullah University of Science and Technology for financial support and Dr. Virginia Unkefer and Dr. Zeyad Al Talla from KAUST and Dr. Christina Morris for their assistance and helpful editorial remarks.

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Authors and Affiliations

  1. Imaging and Characterization Core Lab, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia

    Abdul-Hamid M. Emwas

  2. NMR Core Lab, King Abdullah University of Science and Technology, Room 0149, Thuwal, 23955-6900, Kingdom of Saudi Arabia

    Abdul-Hamid M. Emwas

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  1. Abdul-Hamid M. Emwas
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Correspondence to Abdul-Hamid M. Emwas .

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Editors and Affiliations

  1. Department of Gastroenterology, Medical Section, Herlev Hospital, University of Copenhagen, Copenhagen, Denmark

    Jacob T. Bjerrum

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Emwas, AH.M. (2015). The Strengths and Weaknesses of NMR Spectroscopy and Mass Spectrometry with Particular Focus on Metabolomics Research. In: Bjerrum, J. (eds) Metabonomics. Methods in Molecular Biology, vol 1277. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2377-9_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2377-9_13

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