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Advanced Protocols in Oxidative Stress III pp 379–391Cite as

Liquid Chromatography and Mass Spectrometry for the Analysis of N-β-Methylamino-l-alanine with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1208))

Abstract

Numerous studies in the past decade have identified N-β-methylamino-l-alanine (BMAA) as a putative environmental neurotoxin. Produced by cyanobacteria and accumulated at different levels of the trophic system, BMAA has been detected in the brain tissue of human patients that died from progressive neurodegenerative disease. Research into the mechanism of neurotoxicity has been hampered by conflicting results and disagreement in the literature over analytical methods used for quantification and detection. While several research approaches have been tested, the use of the derivatizing reagent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate is presented here as an effective and selective means for the analysis of BMAA and two co-occurring biological isomers, DAB and AEG, by liquid chromatography and tandem mass spectrometry.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of British Columbia, Kelowna, BC, Canada

    W. Broc Glover, Steven A. Cohen & Susan J. Murch

  2. Department of Chemistry, University of British Columbia, Room 350, Fipke Centre, 3247 University Way, Kelowna, BC, Canada, V1V 1V7

    Susan J. Murch

Authors
  1. W. Broc Glover
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  2. Steven A. Cohen
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  3. Susan J. Murch
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Corresponding author

Correspondence to Susan J. Murch .

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Editors and Affiliations

  1. University of Florida, Gainsville, Florida, USA

    Donald Armstrong

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Glover, W.B., Cohen, S.A., Murch, S.J. (2015). Liquid Chromatography and Mass Spectrometry for the Analysis of N-β-Methylamino-l-alanine with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress III. Methods in Molecular Biology, vol 1208. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1441-8_27

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  • DOI: https://doi.org/10.1007/978-1-4939-1441-8_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1440-1

  • Online ISBN: 978-1-4939-1441-8

  • eBook Packages: Springer Protocols

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