Abstract
Defining the optimal epitope of a CD8 T cell response towards a certain antigen is a multistep procedure that requires the performance of peptide truncation design, ELISPOT peptide titration assays, and assessing the HLA class I restriction of the defined epitope via intracellular cytokine staining assays with B cell lines and epitope-specific CD8 T cell lines.
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References
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© 2014 Springer Science+Business Media, New York
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Roider, J., Vollbrecht, T., Draenert, R. (2014). Mapping of Optimal CD8 T Cell Epitopes. In: Anders, HJ., Migliorini, A. (eds) Innate DNA and RNA Recognition. Methods in Molecular Biology, vol 1169. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0882-0_10
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DOI: https://doi.org/10.1007/978-1-4939-0882-0_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-0882-0
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