Abstract
Since the precise manipulation of DNA often constitutes a fundamental part of biological studies, DNA engineering is a cornerstone of molecular biology. The most commonly used way to engineer DNA relies on the ligation of two individual DNA molecules with the use of restriction sites. Although useful, this method has some limitations which makes it elaborate or even inapplicable for many DNA manipulations. Firstly, it relies on the presence of suitable restriction sites. Suitable restriction enzymes which, for instance, cut uniquely at the desired site of ligation can be hard to find, especially if larger DNA molecules need to be manipulated. Furthermore, due to practical limitations, the engineering of large DNA molecules such as Bacterial Artificial Chromosomes (BACs; 1), P1 vectors (2) and P1 Artificial Chromosomes (PACs; 3) by conventional cloning strategies is virtually impossible.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Shizuya, H., Birren, B., Kim, U. J., Mancico, V., Slepak, T., Tachiiri, Y. and Simon, M. (1992) Proc. Nat. Acad. Sci. U.S.A. 89, 8794–8797.
Sternberg, N. L. (1992) Trends Genet. 8, 11–16.
Ioannou, P. A., Amemiya, C. T., Games, J., Kroisel, P. M., Shizuya, H., Chen, C., Batzer, M. A. and de Jong, P. J. (1994) Nature Genet. 6, 84–89.
Zhang, Y., Buchholz, F., Muyrers, J. P. P. and Stewart, A. F. (1998) Nature Genet. 20, 123–128.
Muyrers, J. P. P., Zhang, Y., Testa, G. and Stewart, A. F. (1999) Nucl. Acids Res. 27, 1555–1557.
Gillen, J. R., Willis, D. K. and Clark, A. J. (1981) J. Bacteriol. 145, 521–532.
Guzman, L., Belin, D., Carson, M. J. and Beckwith, J. (1995) J. Bacteriol. 177, 4121–4130.
Murphy, K. C. (1991) J. Bacteriology 173, 5808–5821.
Muyrers, J. P. P., Zhang, Y. and Stewart, A. F. (unpublished data).
Wagner, M., Zhang, Y., Muyrers, J. P. P. and Stewart, A. F. (unpublished data).
Hashimoto-Gotoh, T. and Sekiguchi, M., (1977) J. Bacteriol. 131, 405–412.
Penfold, R. J. and Pemberton, J. M. (1992) Gene 118, 145–146.
Neidhardt, F. C. (1996) Escherichia coli and Salmonella, Cellular and Molecular Biology, 2nd ed., American Society for Microbiology, Washington, D.C.
Buchholz, F., Angrand, P.-O. and Stewart, A. F. (1996) Nucl. Acids Res. 24, 3118–3119.
Araki, K., Araki, M. and Yamamura, K.-I. (1997) Nucl. Acids Res. 25, 868–872.
Reyrat, J. M., Pelicic, V., Gicquel, B. and Rappuoli, R. (1998) Infect. Immun. 66, 4011–4017.
Blomfield, I. C., Vaughn, V., Rest, R. F. and Eisenstein, B. I. (1991) Mol. Microbiol. 5, 1447–1457.
Angrand, P. O., Daigle, N., van der Hoeven, F., Schöler, H. R. and Stewart, A. F. (1999) Nucl. Acids Res. 27, e16.
Zhang, Y., Muyrers, J. P. P., Testa, G. and Stewart, A. F. (unpublished data).
Shashikant, C. S., Carr, J. L., Bhargava, J., Bentley, K. L. and Ruddle, F. H. (1998) Gene 223, 9–20.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer Science+Business Media New York
About this chapter
Cite this chapter
Muyrers, J.P.P., Zhang, Y., Stewart, A.F. (2000). ET-Cloning: Think Recombination First. In: Setlow, J.K. (eds) Genetic Engineering. Genetic Engineering, vol 22. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4199-8_6
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4199-8_6
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6884-7
Online ISBN: 978-1-4615-4199-8
eBook Packages: Springer Book Archive